成人固定正畸治疗中 2 型糖尿病对龈沟液中前炎症趋化因子谱和糖基化终产物水平的影响。

Effect of type 2 diabetes mellitus in adults undergoing fixed orthodontic treatment on proinflammatory chemokine profile and levels of advanced glycation in gingival crevicular fluid.

机构信息

Department of Orthodontics and Pediatric Dentistry, College of Dentistry, King Khalid University, Abha, Saudi Arabia.

出版信息

Eur Rev Med Pharmacol Sci. 2022 Nov;26(22):8351-8357. doi: 10.26355/eurrev_202211_30369.

DOI:10.26355/eurrev_202211_30369

PMID:36459018

Abstract

OBJECTIVE

The current study aims to reconnoiter the outcome of diabetes mellitus type 2 (DMT2) on levels of advanced glycation end products (AGEs) and pro-inflammatory chemokine in gingival crevicular fluid (GCF) of individuals undergoing fixed orthodontic treatment.

PATIENTS AND METHODS

Participants were divided into a diabetic and no-diabetic group according to inclusion and exclusion criteria. Power analysis was adopted from a previous study that reported GCF chemokines in obese individuals. All teeth were measured for clinical periodontal parameters (CPP). GCF and saliva were collected 3 months after placing stainless steel archwire. GCF was investigated for pro-inflammatory cytokines all expressed in pg/mL. Quantification of chemokines was performed using a Magnetic bead-based multiplex assay for the Luminex® platform. Non-normality of data was assessed by Mann-Whitney U-test. Normality was estimated using an Independent t-test. Descriptive data were computed in the form of standard deviations and means.

RESULTS

Unstimulated whole saliva flow rate (UWSFR) was significantly lower in diabetic patients compared to non-diabetics (p=.021). Amongst different clinical periodontal parameters (CPP) no difference in plaque scores (PS) and probing depth (PD) was found between diabetic and non-diabetic participants. Two GCF chemokines i.e., Resistin (p=.031) and AGEs (p=.017) were observed to be significantly higher in DMT2 participants compared to the non-diabetes group. CPP and GCF biomarkers in diabetic and non-diabetic individuals demonstrated a positive correlation between AGEs and GCF resistin levels concerning bleeding on probing (BoP) in diabetic patients.

CONCLUSIONS

Participants with DMT2 after alignment with an orthodontic device exhibited significantly high levels of resistin and AGEs. The proinflammatory response was noted in patients with hyperglycemia undergoing orthodontic treatment.

摘要

目的

本研究旨在探讨 2 型糖尿病（DMT2）对接受固定正畸治疗个体牙龈沟液（GCF）中晚期糖基化终产物（AGEs）和促炎趋化因子水平的影响。

患者和方法

根据纳入和排除标准，将参与者分为糖尿病组和非糖尿病组。采用之前一项研究报告的肥胖个体 GCF 趋化因子的功效分析。所有牙齿均测量临床牙周参数（CPP）。不锈钢弓丝放置 3 个月后收集 GCF 和唾液。GCF 用于检测以 pg/mL 表示的促炎细胞因子。使用基于磁珠的多重测定法对 Luminex®平台进行趋化因子定量。使用 Mann-Whitney U 检验评估数据的非正态性。使用独立 t 检验估计正态性。以标准差和平均值的形式计算描述性数据。

结果

与非糖尿病患者相比，糖尿病患者的未刺激全唾液流速（UWSFR）明显较低（p=.021）。在不同的临床牙周参数（CPP）中，糖尿病和非糖尿病参与者的菌斑评分（PS）和探诊深度（PD）没有差异。两种 GCF 趋化因子，即抵抗素（p=.031）和 AGEs（p=.017），在 DMT2 参与者中观察到明显高于非糖尿病组。糖尿病和非糖尿病个体的 CPP 和 GCF 生物标志物显示 AGEs 与 GCF 抵抗素水平之间存在正相关，与糖尿病患者探诊出血（BoP）有关。