Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China.
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, College of Life Science, Dalian Minzu University, Dalian 116600, China.
Food Chem. 2023 Apr 16;406:134663. doi: 10.1016/j.foodchem.2022.134663. Epub 2022 Oct 19.
Surface browning negatively impacts the shelf-life of fresh-cut apple. Herein, we found that the browning of fresh-cut apple aggravated rapidly after 24 h post-cutting, then the transcriptomic and miRNA expression profiles of fresh-cut apple immediately after cutting (T0) and 24 h post-cutting (T24) were analyzed to explore the molecular mechanism of early browning response. A total of 3156 differentially expressed mRNAs (DEGs) and 23 differentially expressed miRNAs (DEmiRNAs) were identified in T24 vs T0. Most DEGs related to respiratory, energy, antioxidant, lipid and secondary metabolism were activated in the early stage of browning. There were 63 target genes of 10 DEmiRNAs validated by degradome sequencing and among them, mdm-miR156aa_L + 1_1 targets 12-oxophytodienoate reductase, ptc-miR6478_R-1 targets patatin-like protein, mdm-miR156aa_L + 1_1 and mdm-miR156aa_L + 1_2 co-target SPLs might participate in the early browning response through regulating antioxidant, lipid and secondary metabolism. Our results will be beneficial for the technological innovation of browning amelioration for fresh-cut apple.
果皮褐变会显著降低鲜切苹果的货架期。在这里，我们发现鲜切苹果在切割后 24 小时内迅速加剧褐变，然后立即分析鲜切苹果切割后即刻（T0）和 24 小时后（T24）的转录组和 miRNA 表达谱，以探讨早期褐变反应的分子机制。在 T24 与 T0 相比，共鉴定出 3156 个差异表达的 mRNAs（DEGs）和 23 个差异表达的 miRNA（DEmiRNAs）。大多数与呼吸、能量、抗氧化、脂质和次生代谢相关的 DEGs 在褐变的早期阶段被激活。通过降解组测序验证了 10 个 DEmiRNAs 的 63 个靶基因，其中 mdm-miR156aa_L+1_1 靶标 12-氧代植物二烯酸还原酶，ptc-miR6478_R-1 靶标类脂酶，mdm-miR156aa_L+1_1 和 mdm-miR156aa_L+1_2 共同靶标 SPLs 可能通过调节抗氧化、脂质和次生代谢参与早期褐变反应。我们的研究结果将有利于鲜切苹果褐变改良的技术创新。
