Zhang Jialin, Zhou Ming, Li Xin, Fan Yaqi, Li Jinhui, Lu Kangqiang, Wen Herui, Ren Jiali
Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry, School of Chemistry and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou, 341000, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Jiangxi Provincial Key Laboratory of Functional Molecular Materials Chemistry, School of Chemistry and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou, 341000, PR China.
Talanta. 2023 Mar 1;254:124133. doi: 10.1016/j.talanta.2022.124133. Epub 2022 Nov 25.
Bacterial infections have become a global public health problem. Rapid and sensitive bacterial detection is of great importance for human health. Among various sensor systems, fluorescence sensor is rapid, portable, multiplexed, and cost-efficient. Herein, we reviewed the current trends of fluorescent sensors for bacterial detection from three aspects (response materials, target and recognition way). The fluorescent materials have the advantages of high fluorescent strength, high stability, and good biocompatibility. They provide a new path for bacterial detection. Several recent fluorescent nanomaterials for bacterial detection, including semiconductor quantum dots (QDs), carbon dots (CDs), up-conversion nanoparticles (UCNPs) and metal organic frameworks (MOFs), were introduced. Their optical properties and detection mechanisms were analyzed and compared. For different response targets in the detection process, we studied the fluorescence strategy using DNA, bacteria, and metabolites as the response target. In addition, we classified the recognition way between nanomaterial and target, including specific recognition methods based on aptamers, antibodies, bacteriophages, and non-specific recognition methods based on biological functional materials. The characteristics of different recognition methods were summarized. Finally, the weaknesses and future development of bacterial fluorescence sensor were discussed. This review provides new insights into the application of fluorescent sensing systems as an important tool for bacterial detection.
细菌感染已成为一个全球公共卫生问题。快速且灵敏的细菌检测对人类健康至关重要。在各种传感器系统中，荧光传感器具有快速、便携、可多重检测且成本效益高的特点。在此，我们从响应材料、目标物及识别方式三个方面综述了用于细菌检测的荧光传感器的当前发展趋势。荧光材料具有荧光强度高、稳定性好及生物相容性佳的优点。它们为细菌检测提供了一条新途径。介绍了几种近期用于细菌检测的荧光纳米材料，包括半导体量子点（QDs）、碳点（CDs）、上转换纳米粒子（UCNPs）和金属有机框架（MOFs）。分析并比较了它们的光学性质和检测机制。针对检测过程中的不同响应目标物，我们研究了以DNA、细菌和代谢物作为响应目标物的荧光策略。此外，我们对纳米材料与目标物之间的识别方式进行了分类，包括基于适配体、抗体、噬菌体的特异性识别方法以及基于生物功能材料的非特异性识别方法。总结了不同识别方法的特点。最后，讨论了细菌荧光传感器的不足及未来发展方向。本综述为荧光传感系统作为细菌检测的重要工具的应用提供了新的见解。
