Song Shiyu, Han Lu, Chen Min, Pan Leiqing, Tu Kang
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Sensors (Basel). 2024 Dec 2;24(23):7715. doi: 10.3390/s24237715.
Food safety is of great concern, and food-borne bacterial infections and diseases are a major crisis for health. Therefore, it is necessary to develop rapid detection techniques for the prevention and recognition of food safety hazards caused by food-borne pathogens. In recent years, the fluorescence assay has become a widely utilized detection method due to its good signal amplification effect, high detection sensitivity, high stability, and short detection time. In this review, the different kinds of fluorescence materials were concentrated, including quantum dots (QDs), carbon dots (CDs), metal-organic frameworks (MOFs), and upconversion nanoparticles (UCNPs). The optical properties and applications of different kinds of fluorescent materials were analyzed and compared. Furthermore, according to the biosensing components, different fluorescence biosensors are reviewed, including label-free based fluorescence probes, aptamer-based biosensors, and antibody-based biosensors. Finally, we focused our attention on the discussion of fluorescent detection techniques combined with other techniques and their applications. The review presents future trends in fluorescence sensors, providing new sights for the detection of food-borne pathogens.
食品安全备受关注,食源性细菌感染和疾病是健康面临的重大危机。因此,有必要开发快速检测技术,以预防和识别食源性病原体引起的食品安全危害。近年来,荧光分析法因其良好的信号放大效果、高检测灵敏度、高稳定性和短检测时间,已成为一种广泛应用的检测方法。在这篇综述中,集中介绍了不同种类的荧光材料,包括量子点(QDs)、碳点(CDs)、金属有机框架(MOFs)和上转换纳米粒子(UCNPs)。分析并比较了不同种类荧光材料的光学性质和应用。此外,根据生物传感组件,综述了不同的荧光生物传感器,包括无标记荧光探针、适体生物传感器和抗体生物传感器。最后,我们重点讨论了荧光检测技术与其他技术的结合及其应用。该综述展示了荧光传感器的未来发展趋势,为食源性病原体的检测提供了新的视角。