Wusteman M C, Jacobsen I A, Pegg D E
Scand J Urol Nephrol. 1978;12(3):281-6. doi: 10.3109/00365597809179731.
There is good evidence that blood should be removed from kidneys as completely as possible before they are transplanted. Rabbit kidneys, either freshly isolated or after one hour of warm ischaemia, were perfused with a solution of osmolality 400 mOsm/kg which contained high concentrations of Mg++ (72 mEq) and glucose (167 mM). The rate and completeness of blood removal were measured by collecting effluent fractions until 100 ml had been perfused, and then assaying the haemoglobin in each effluent fraction and the residual haemoglobin in the kidney. A pressure of 60 mmHg was found to be sufficient for effective perfusion. The addition of 5% dextran 40 (Rheomacrodex) to the perfusate greatly improved the completeness of blood removal from kidneys subjected to one hour of warm ischaemia. Rabbit kidneys washed out with this solution functioned excellently after transplantation.
有充分证据表明，在肾脏移植前应尽可能彻底地清除其中的血液。对新鲜分离的兔肾或经历1小时热缺血后的兔肾，用渗透压为400 mOsm/kg的溶液进行灌注，该溶液含有高浓度的Mg++（72 mEq）和葡萄糖（167 mM）。通过收集流出液部分直至灌注100 ml，然后测定每个流出液部分中的血红蛋白以及肾脏中的残留血红蛋白，来测量血液清除的速率和彻底程度。发现60 mmHg的压力足以进行有效灌注。向灌注液中添加5%的右旋糖酐40（低分子右旋糖酐）可大大提高经历1小时热缺血的肾脏的血液清除彻底程度。用该溶液冲洗后的兔肾在移植后功能良好。
