College of Life Sciences, Yantai University, Yantai, Shandong China.
Yantai Lanchuang Biotechnology Co., Ltd., Yantai, Shandong China.
Int J Artif Organs. 2023 Feb;46(2):99-112. doi: 10.1177/03913988221139883. Epub 2022 Dec 5.
Nile tilapia (hereinafter referred to as tilapia) is a species with high economic value and extensive cultivation. In this study, the low-temperature Nile tilapia skin collagen powder (TSCP) was prepared by liquid nitrogen freeze pulverization. After physical and chemical analysis of its properties, it was found that its characteristics were similar to those of type I collagen. The three-dimensional helix structure of protein peptide is good and non denatured. It shows that cryogenic temperature guarantees the activity of TSCP. In addition, TSCP has good biocompatibility. Specifically, it has good blood compatibility, lacks cytotoxicity, will not cause intradermal stimulation and acute systemic toxicity, and has no obvious rejection after implantation. In the rat liver hemorrhage model and wound repair model, compared with the commercially available bovine collagen powder (BSCP), TSCP has better blood coagulation ability: the shortest hemostatic time (135 s) and wound healing efficiency: the wound healing is obvious on the 14th day. The results of this study indicate that the TSCP is an ideal candidate for hemostatic agents and wound healing dressings.
罗非鱼（以下简称罗非鱼）是一种经济价值高、养殖范围广的物种。本研究采用液氮冷冻粉碎法制备低温罗非鱼皮胶原蛋白粉（TSCP）。通过对其性能的理化分析，发现其特性与 I 型胶原蛋白相似。其蛋白质肽的三维螺旋结构良好，未变性。这表明低温保证了 TSCP 的活性。此外，TSCP 具有良好的生物相容性。具体来说，它具有良好的血液相容性，无细胞毒性，不会引起皮内刺激和急性全身毒性，植入后无明显排斥反应。在大鼠肝出血模型和伤口修复模型中，与市售牛胶原蛋白粉（BSCP）相比，TSCP 具有更好的凝血能力：最短的止血时间（135 秒）和伤口愈合效率：第 14 天伤口愈合明显。本研究结果表明，TSCP 是一种理想的止血剂和伤口愈合敷料的候选材料。
