Department of Cosmetic and Plastic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266071, China.
Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250000, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 4):127014. doi: 10.1016/j.ijbiomac.2023.127014. Epub 2023 Sep 22.
Hemostasis plays a critical role in the early stage of wound healing, especially in acute wounds which can significantly improve the survival of patients. Based on the excellent biocompatibility of natural biomaterials, in this study, we prepared a series of novel hemostatic sponges by using tilapia skin, a marine biological resource, and extracting tilapia skin-derived gelatin, collagen, and acellular dermal matrix through five different methods. Using in vitro sheep blood and in vivo rat liver hemorrhage models, we found that tilapia skin sponges had excellent coagulation and hemostatic abilities. Among them, the collagen sponge exhibited optimal hemostasis performance because it could accelerate clotting by binding to the specific sites of blood cells and platelets. Furthermore, the sponges' porous structure enhanced the capability to absorb blood, thus effectively promoting hemostasis. In summary, we reported an efficient and convenient method to prepare marine biological resources into sponges, which provided a novel class of alternatives for hemostasis in acute wounds with broad application prospects.
止血在伤口愈合的早期阶段起着至关重要的作用，特别是在急性伤口中，可以显著提高患者的生存率。基于天然生物材料优异的生物相容性，本研究利用海洋生物资源罗非鱼皮，通过五种不同方法提取罗非鱼皮来源的明胶、胶原蛋白和脱细胞真皮基质，制备了一系列新型止血海绵。通过体外绵羊血和体内大鼠肝出血模型，发现罗非鱼皮海绵具有优异的凝血和止血能力。其中，胶原蛋白海绵表现出最佳的止血性能，因为它可以通过与血细胞和血小板的特定结合位点结合来加速凝血。此外，海绵的多孔结构增强了吸收血液的能力，从而有效地促进止血。总之，我们报道了一种将海洋生物资源制备成海绵的高效便捷方法，为急性伤口的止血提供了一类新的替代品，具有广阔的应用前景。
