Xu Chunlan, Guo Yu, Qiao Lei, Ma Li, Cheng Yiyi, Roman Alexandra
The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, China.
Front Microbiol. 2018 Jun 18;9:1129. doi: 10.3389/fmicb.2018.01129. eCollection 2018.
Selenium (Se) is an essential element for human and animal health. Biogenic selenium nanoparticles (SeNPs) by microorganism possess unique physical and chemical properties and biological activities compared with inorganic Se and organic Se. The study was conducted to investigate the mainly biological activities of SeNPs by ATCC 393 ( 393). The results showed that 393 transformed sodium selenite to red SeNPs with the size of 50-80 nm, and accumulated them intracellularly. 393-SeNPs promoted the growth and proliferation of porcine intestinal epithelial cells (IPEC-J2), human colonic epithelial cells (NCM460), and human acute monocytic leukemia cell (THP-1)-derived macrophagocyte. 393-SeNPs significantly inhibited the growth of human liver tumor cell line-HepG2, and alleviated diquat-induced IPEC-J2 oxidative damage. Moreover, and experimental results showed that administration with 393-SeNPs protected against Enterotoxigenic K88 (ETEC K88)-caused intestinal barrier dysfunction. ETEC K88 infection-associated oxidative stress (glutathione peroxidase activity, total superoxide dismutase activity, total antioxidant capacity, and malondialdehyde) was ameliorated in 393-SeNPs-treated mice. These findings suggest that 393-SeNPs with no cytotoxicity play a key role in maintaining intestinal epithelial integrity and intestinal microflora balance in response to oxidative stress and infection.
硒（Se）是人和动物健康所必需的元素。与无机硒和有机硒相比，微生物产生的生物源硒纳米颗粒（SeNPs）具有独特的物理化学性质和生物活性。本研究旨在探究ATCC 393（393）产生的SeNPs的主要生物活性。结果表明，393可将亚硒酸钠转化为尺寸为50 - 80 nm的红色SeNPs，并将其积累在细胞内。393 - SeNPs促进了猪肠上皮细胞（IPEC - J2）、人结肠上皮细胞（NCM460）以及人急性单核细胞白血病细胞（THP - 1）来源的巨噬细胞的生长和增殖。393 - SeNPs显著抑制人肝癌细胞系 - HepG2的生长，并减轻百草枯诱导的IPEC - J2氧化损伤。此外，实验结果表明，给予393 - SeNPs可预防产肠毒素大肠杆菌K88（ETEC K88）引起的肠道屏障功能障碍。在393 - SeNPs处理的小鼠中，ETEC K88感染相关的氧化应激（谷胱甘肽过氧化物酶活性、总超氧化物歧化酶活性、总抗氧化能力和丙二醛）得到改善。这些发现表明，无细胞毒性的393 - SeNPs在应对氧化应激和感染时，在维持肠道上皮完整性和肠道微生物群平衡方面发挥着关键作用。
