Örenli Demet, Kaçar Selvi Ceren, Öztürk Funda, Erden Pınar Esra, Kılıç Esma
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
Department of Chemistry, Faculty of Science and Arts, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Anal Biochem. 2023 Feb 1;662:115002. doi: 10.1016/j.ab.2022.115002. Epub 2022 Dec 5.
Electrochemical (bio)sensors were developed for bisphenol A (BPA) determination. Screen printed carbon electrode (SPCE) was modified with ionic liquid 1- butyl-3-methylimidazolium tetrafluoroborate (IL), carbon quantum dots (CQD) and gold nanoparticles (AuNP) for the fabrication of the BPA sensor. Electrode surface composition was optimized for the deposition time of AuNP, amount of CQD and percentage of IL using the central composite design (CCD) method. The results of the CCD study indicated that maximum amperometric response was recorded when 9.8 μg CQD, 3% IL and 284 s AuNP deposition time were used in modification. Tyrosinase (Ty) was further modified on the AuNP/CQD-IL/SPCE to fabricate the biosensor. Analytical performance characteristics of the BPA sensor were investigated by differential pulse anodic adsorptive stripping voltammetry and the AuNP/CQD-IL/SPCE sensor exhibited a linear response to BPA in the range of 2.0 × 10 - 3.6 × 10 M with a detection limit of 1.1 × 10 M. Amperometric measurements showed that the linear dynamic range and detection limit of the Ty/AuNP/CQD-IL/SPCE were 2.0 × 10 - 4.0 × 10 M and 6.2 × 10 M, respectively. Analytical performance characteristics such as sensitivity, reproducibility and selectivity were investigated for the presented (bio)sensors. The analytical applicability of the (bio)sensors to the analysis of BPA in mineral water samples was also tested.
开发了用于测定双酚A（BPA）的电化学（生物）传感器。采用离子液体1-丁基-3-甲基咪唑四氟硼酸盐（IL）、碳量子点（CQD）和金纳米颗粒（AuNP）对丝网印刷碳电极（SPCE）进行修饰，以制备BPA传感器。采用中心复合设计（CCD）方法，针对AuNP的沉积时间、CQD的用量和IL的百分比对电极表面组成进行了优化。CCD研究结果表明，修饰时使用9.8μg CQD、3% IL和284 s的AuNP沉积时间可记录到最大安培响应。在AuNP/CQD-IL/SPCE上进一步修饰酪氨酸酶（Ty）以制备生物传感器。采用差分脉冲阳极吸附溶出伏安法研究了BPA传感器的分析性能特征，AuNP/CQD-IL/SPCE传感器对BPA在2.0×10 - 3.6×10 M范围内呈现线性响应，检测限为1.1×10 M。安培测量表明，Ty/AuNP/CQD-IL/SPCE的线性动态范围和检测限分别为2.0×10 - 4.0×10 M和6.2×10 M。研究了所提出的（生物）传感器的灵敏度、重现性和选择性等分析性能特征。还测试了（生物）传感器对矿泉水样品中BPA分析的分析适用性。
