Department of Chemistry, Polatlı Faculty of Science and Letters, Ankara Haci Bayram Veli University, Ankara, Türkiye.
Department of Chemistry, Polatlı Faculty of Science and Letters, Ankara Haci Bayram Veli University, Ankara, Türkiye.
Anal Biochem. 2024 May;688:115464. doi: 10.1016/j.ab.2024.115464. Epub 2024 Jan 18.
This study describes the development of a highly sensitive amperometric biosensor for the analysis of phenolic compounds such as catechol. The biosensor architecture is based on the immobilization of tyrosinase (Tyr) on a screen-printed carbon electrode (SPE) modified with nanodiamond particles (ND), 1-butyl-3-methylimidazolium hexafluorophosphate (IL) and poly-l-lysine (PLL). Surface morphologies of the electrodes during the modification process were evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to investigate the electrochemical characteristics of the modified electrodes. Owing to the synergistic effect of the modification materials, the Tyr/PLL/ND-IL/SPE exhibited high sensitivity (328.2 μA mM) towards catechol with a wide linear range (5.0 × 10 - 1.2 × 10 M) and low detection limit (1.1 × 10 M). Furthermore, the method demonstrated good reproducibility and stability. The amperometric response of the biosensor towards other phenolic compounds such as bisphenol A, phenol, p-nitrophenol, m-cresol, p-cresol and o-cresol was also investigated. The analytical applicability of the biosensor was tested by the analysis of catechol in tap water. The results of the tap water analysis showed that the Tyr/PLL/ND-IL/SPE can be used as a practical and effective method for catechol determination.
本研究描述了一种用于分析儿茶酚等酚类化合物的高灵敏度电流生物传感器的开发。该生物传感器的结构基于将酪氨酸酶（Tyr）固定在纳米金刚石颗粒（ND）、1-丁基-3-甲基咪唑六氟磷酸盐（IL）和聚-l-赖氨酸（PLL）修饰的丝网印刷碳电极（SPE）上。通过扫描电子显微镜（SEM）和能谱（EDX）评估了电极在修饰过程中的表面形态。循环伏安法（CV）和电化学阻抗谱（EIS）用于研究修饰电极的电化学特性。由于修饰材料的协同作用，Tyr/PLL/ND-IL/SPE 对儿茶酚表现出高灵敏度（328.2 μA mM），线性范围宽（5.0×10 - 1.2×10 M），检测限低（1.1×10 M）。此外，该方法还表现出良好的重现性和稳定性。该生物传感器对其他酚类化合物（如双酚 A、苯酚、对硝基苯酚、间甲酚、对甲酚和邻甲酚）的电流响应也进行了研究。通过对自来水中儿茶酚的分析，测试了生物传感器的分析适用性。自来水中儿茶酚分析的结果表明，Tyr/PLL/ND-IL/SPE 可作为一种实用有效的儿茶酚测定方法。
