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(昆斯)杰索普的完整叶绿体基因组序列:天门冬科的基因组结构、基因组成及系统发育分析

The entire chloroplast genome sequence of (Kunth) Jessop: Genome structure, gene composition, and phylogenetic analysis in Asparagaceae.

作者信息

Kuang Quan, Sheng Wentao

机构信息

Department of Biological Technology, Nanchang Normal University, Nanchang, 330032, Jiangxi, China.

出版信息

Open Life Sci. 2022 Nov 23;17(1):1541-1554. doi: 10.1515/biol-2022-0497. eCollection 2022.

Abstract

(Kunth) Jessop is a horticultural plant of the genus . Herein, the whole chloroplast (cp) genome of was sequenced with PacBio and Illumina sequencing systems. The cp genome shows a characteristic quadripartite structure with 158,076 bp. In total, 135 genes were annotated, containing 89 protein-coding, 38 tRNA, and 8 rRNA genes. Contrast with the previous cp genome of registered in NCBI, we identified 7 single-nucleotide polymorphisms and 15 indels, mostly situated in noncoding areas. Meanwhile, 36 repeat structures and 260 simple sequence repeats were marked out. A bias for A/T-ending codons was shown in this cp genome. Furthermore, we predicted 78 RNA-editing sites in 29 genes, which were all for C-to-U transitions. And it was also proven that positive selection was exerted on the gene of with the / data. Meanwhile, a conservative gene order and highly similar sequences of protein-coding genes were revealed within species. Phylogenetic tree analysis indicated that was a sister to . Taken together, our released genome provided valuable information for the gene composition, genetics comparison, and the phylogeny studies of .

摘要

(昆斯)杰索普是该属的一种园艺植物。在此,利用PacBio和Illumina测序系统对其完整叶绿体(cp)基因组进行了测序。该cp基因组呈现出具有158,076 bp的典型四分体结构。总共注释了135个基因,包括89个蛋白质编码基因、38个tRNA基因和8个rRNA基因。与NCBI中注册的该植物先前的cp基因组相比,我们鉴定出7个单核苷酸多态性和15个插入缺失,大多位于非编码区。同时,标记出36个重复结构和260个简单序列重复。该cp基因组显示出对以A/T结尾密码子的偏好。此外,我们在29个基因中预测了78个RNA编辑位点,均为C到U的转换。并且利用Ka/Ks数据也证明了杰索普的基因受到正选择。同时,在该植物物种内揭示了保守的基因顺序和蛋白质编码基因的高度相似序列。系统发育树分析表明杰索普是[另一植物名称]的姐妹种。总之,我们发布的基因组为杰索普的基因组成、遗传比较和系统发育研究提供了有价值的信息。

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