ACE2受体多态性及其与抗SARS-CoV-2 IgM抗体的相关性——一项病例对照研究。
ACE2 Receptor Polymorphism and its Correlation with Anti-SARS-CoV-2 IgM Antibodies - A Case-Control Study.
作者信息
Siraj Anum, Yahya Saira, Khan Amber, Awan Saleem
机构信息
Department of Biosciences, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology (SZABIST), 100-Clifton Karachi, Pakistan.
出版信息
Protein Pept Lett. 2023;30(2):146-153. doi: 10.2174/0929866530666221206094926.
BACKGROUND
The SARS-CoV-2 pandemic originated in Wuhan, China in December 2019 and spread rapidly worldwide. The virus gets entry into target cells via angiotensin-converting enzyme 2 (ACE2) receptors and its gene is highly polymorphic.
INTRODUCTION
The variations in SARS-CoV-2 susceptibility and severity can be explained on a genetic level by studying the polymorphism in ACE2 receptor polymorphism.
OBJECTIVE
A prospective case-control study was designed to compare the ACE2 levels in SARS-CoV- 2 patients with the healthy controls in the local population, for which a total of 100 EDTA-containing blood samples were included (50 SARS-CoV-2 IgM positive case and 50 healthy controls).
METHODS
PCR-RFLP was performed to investigate the polymorphism of ACE2 in genomic DNA and the ACE2 plasma levels were determined through ELISA.
RESULTS
No significant difference in allelic and genotype frequencies (GG, GA, AA) were observed while the ACE2 plasma levels were found to be decreased in positive samples.
CONCLUSION
No significant association of the ACE2 gene polymorphism (G8790A) was found with the SARS-CoV-2 susceptibility in the Pakistani population which intimates the search for other genetic factors within the local population.
背景
2019年12月，严重急性呼吸综合征冠状病毒2（SARS-CoV-2）大流行起源于中国武汉，并迅速在全球传播。该病毒通过血管紧张素转换酶2（ACE2）受体进入靶细胞，其基因具有高度多态性。
引言
通过研究ACE2受体多态性，可在基因水平上解释SARS-CoV-2易感性和严重程度的差异。
目的
设计一项前瞻性病例对照研究，比较当地人群中SARS-CoV-2患者与健康对照者的ACE2水平，共纳入100份含乙二胺四乙酸（EDTA）的血样（50例SARS-CoV-2 IgM阳性病例和50例健康对照）。
方法
采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）研究基因组DNA中ACE2的多态性，并通过酶联免疫吸附测定（ELISA）测定血浆ACE2水平。
结果
等位基因和基因型频率（GG、GA、AA）无显著差异，但阳性样本中血浆ACE2水平降低。
结论
在巴基斯坦人群中，未发现ACE2基因多态性（G8790A）与SARS-CoV-2易感性有显著关联，这提示需在当地人群中寻找其他遗传因素。
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