Department of Urology, Söke Fehime Faik Kocagöz State Hospital, Aydin, Turkey.
Department of Medical Biochemistry, Adnan Menderes University School of Medicine, Aydin, Turkey.
Low Urin Tract Symptoms. 2023 Mar;15(2):63-67. doi: 10.1111/luts.12470. Epub 2022 Dec 7.
To reveal the histopathological and immunological outcomes of intravesical treatment with tarantula cubensis extract (TCE) in a rat model of interstitial cystitis.
A total of 30 female Wistar albino rats were divided into three groups: group 1 (control group), group 2 (disease group), and group 3 (treatment group). The rat model of interstitial cystitis was created by biweekly intraperitoneal administration of cyclophosphamide (CYP). In group 3, TCE (a venom extracted from a brown spider known as tarantula cubensis) was administered intravesically after the model had been created. Urothelial degeneration, necrosis, ulcer, bleeding, edema, inflammation and mast cell count, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), myeloperoxidase (MPO), and hydroxyproline parameters were evaluated. Statistical analysis was performed using one-way analysis of variance, chi-square tests, and Kruskal-Wallis tests.
All parameters were found to be lower in the rats in group 1 than in the other groups, and IL-6 and MPO values were found to be higher in group 2 (p < .001). The mean TNF-alpha value was highest in group 2 (p = .078). No difference was found between all groups regarding ulcer (p = .087). Urothelial degeneration, necrosis, edema, inflammation, hemorrhage and fibroblast proliferations, and hydroxyproline values were higher in group 3 (p < .001).
Intravesical TCE instillation produces an anti-inflammatory effect by reducing the levels of inflammatory parameters such as IL-6, TNF-alpha, and MPO in bladder tissue. It also accelerates tissue healing by increasing hydroxyproline and fibroblast proliferation.
揭示 Tarantula cubensis 提取物（TCE）膀胱内治疗在间质性膀胱炎大鼠模型中的组织病理学和免疫学结果。
将 30 只雌性 Wistar 白化大鼠分为三组：第 1 组（对照组）、第 2 组（疾病组）和第 3 组（治疗组）。通过每周两次腹腔内给予环磷酰胺（CYP）建立大鼠间质性膀胱炎模型。在第 3 组中，在建立模型后经膀胱内给予 TCE（一种从名为 Tarantula cubensis 的棕色蜘蛛中提取的毒液）。评估尿路上皮变性、坏死、溃疡、出血、水肿、炎症和肥大细胞计数、白细胞介素 6（IL-6）、肿瘤坏死因子-α（TNF-α）、髓过氧化物酶（MPO）和羟脯氨酸参数。使用单向方差分析、卡方检验和 Kruskal-Wallis 检验进行统计分析。
第 1 组的所有参数均低于其他组，且第 2 组的 IL-6 和 MPO 值更高（p < .001）。第 2 组的平均 TNF-α值最高（p=0.078）。各组之间的溃疡无差异（p=0.087）。第 3 组的尿路上皮变性、坏死、水肿、炎症、出血和纤维母细胞增殖以及羟脯氨酸值更高（p < .001）。
膀胱内 TCE 灌注通过降低膀胱组织中炎症参数（如 IL-6、TNF-α 和 MPO）的水平产生抗炎作用。它还通过增加羟脯氨酸和纤维母细胞增殖来加速组织愈合。
