Nielsen P L
Acta Pathol Microbiol Scand B. 1978 Dec;86B(6):321-6. doi: 10.1111/j.1699-0463.1978.tb00051.x.
The uptake of tritium-labelled streptomycin by cells of Eschericia coli K 12 was shown to be only partly and transiently inhibited by the reported antagonists cyanide and chloramphenicol. After a lag, uptake of streptomycin took place in the presence of cyanide, although at a decreased rate. The lag was absent when cells were treated with cyanide some time before streptomycin. The cyanide-resistant transport system showed the same sensitivity to salt as the normal system. By increasing the salt content of the complex medium used, the uptake rate was decreased and several different phases in the uptake became detectable, including an early saturation phase of unknown nature. Uptake in a mineral salt medium was compared with that in complex medium, and differences in uptake were found explainable by differences in salt content. Chloramphenicol, in a concentration of 50 microgram/ml, was shown to permit an uptake rate (after a lag) of about one-fifth of the normal uptake rate in the complex medium. When the last rapid uptake phase, coincident with killing, was delayed by salt or cyanide, chloramphenicol had little or no effect on uptake. At higher concentrations, it enhanced the uptake and caused lysis of the bacteria. Based on the inhibition pattern produced by the inhibitors mentioned above, both alone and in combination, a hypothesis for the uptake of streptomycin by Escherichia coli is submitted.
已表明，氰化物和氯霉素这两种报告的拮抗剂仅部分且短暂地抑制了大肠杆菌K12细胞对氚标记链霉素的摄取。经过一段延迟后，在存在氰化物的情况下仍会发生链霉素的摄取，尽管摄取速率有所降低。如果在加入链霉素之前一段时间先用氰化物处理细胞，则不会出现延迟现象。抗氰化物转运系统对盐的敏感性与正常系统相同。通过增加所用复合培养基的盐含量，摄取速率降低，并且在摄取过程中可检测到几个不同阶段，包括一个性质不明的早期饱和阶段。将在无机盐培养基中的摄取与在复合培养基中的摄取进行了比较，发现摄取差异可以用盐含量的差异来解释。浓度为50微克/毫升的氯霉素显示，（经过一段延迟后）摄取速率约为复合培养基中正常摄取速率的五分之一。当与杀菌同时发生的最后一个快速摄取阶段因盐或氰化物而延迟时，氯霉素对摄取几乎没有影响或没有影响。在较高浓度下，它会增强摄取并导致细菌裂解。基于上述抑制剂单独或联合产生的抑制模式，提出了大肠杆菌摄取链霉素的假说。
