Zhang Minghui, Liu Kunlun
College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.
School of Food and Reserves Storage, Henan University of Technology, Zhengzhou 450001, China.
Foods. 2022 Dec 1;11(23):3878. doi: 10.3390/foods11233878.
Selenium-rich rice has become one of the effective ways to increase people's selenium intake. Selenium-containing proteins have higher antioxidant properties, which may lead to selenium-rich brown rice (Se-BR) having better storage stability than ordinary brown rice (BR). By measuring the peroxidation value, fatty acid value, carbonyl value and protein secondary structure, it was found that Se-BR had higher oxidation resistance stability than BR. The biological function of the differential proteins (DEPs) between ordinary brown rice stored for 0 days (BR-0) and 180 days (BR-6) as well as Se-rich brown rice stored for 0 days (Se-0) and 180 days (Se-6) was investigated by using iTRAQ. A total of 237, 235, 113 and 213 DEPs were identified from group A (BR-0/BR-6), group B (Se-0/Se-6), group C (BR-0/Se-0) and group D (BR-6/Se-6), respectively. Kyoto Encyclopedia of Genes and Genomes analysis showed that the DEPs were mainly enriched in glucose metabolism, tricarboxylic acid cycle, fatty acid biosynthesis and degradation, glutathione metabolism, sulfur metabolism, peroxisome and other metabolic pathways. This study provides theoretical support for the study of protein oxidation kinetics and storage quality control of brown rice during storage.
富硒大米已成为增加人们硒摄入量的有效途径之一。含硒蛋白质具有较高的抗氧化性能,这可能导致富硒糙米(Se-BR)比普通糙米(BR)具有更好的储存稳定性。通过测定过氧化值、脂肪酸值、羰基值和蛋白质二级结构,发现Se-BR比BR具有更高的抗氧化稳定性。采用iTRAQ技术研究了普通糙米储存0天(BR-0)和180天(BR-6)以及富硒糙米储存0天(Se-0)和180天(Se-6)之间差异蛋白质(DEPs)的生物学功能。分别从A组(BR-0/BR-6)、B组(Se-0/Se-6)、C组(BR-0/Se-0)和D组(BR-6/Se-6)中鉴定出237、235、113和213个DEPs。京都基因与基因组百科全书分析表明,这些DEPs主要富集在糖代谢、三羧酸循环、脂肪酸生物合成与降解、谷胱甘肽代谢、硫代谢、过氧化物酶体等代谢途径中。本研究为糙米储存期间蛋白质氧化动力学及储存品质控制的研究提供了理论支持。