Hurdogan Ozge, De Logu Francesco, Galli Francesca, Tuncer Samuray, Ugolini Filippo, Simi Sara, Portelli Francesca, Nassini Romina, Massi Daniela, Buyukbabani Nesimi
Department of Pathology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey.
Department of Health Sciences, Section of Clinical Pharmacology and Oncology, University of Florence, 50100 Florence, Italy.
Cancers (Basel). 2022 Dec 2;14(23):5959. doi: 10.3390/cancers14235959.
Although it is a disease that occurs mainly in the Caucasian population, uveal melanoma (UM) is the most common primary intraocular tumor in adults. Here, we used digital pathology and image analysis for the diagnosis of UM and the prediction of the prognosis. Our retrospective study included a total of 404 histopathological slides from 101 patients. A digital image acquisition and quantitative analysis of tissue immune biomarkers (CD4, CD8, CD68, CD163) were performed. A negative impact of the intratumoral CD8 positive cell density higher than 13.3 cells/mm was detected for both RFS (HR 2.08, 95% Cl 1.09 to 3.99, = 0.027) and OS (HR 3.30, 95% CI 1.58 to 6.88, = 0.001). Moreover, we confirmed that older age and stage III were independent negative prognostic factors for both RFS and OS. Our results suggest that a specific distribution profile of CD8 in UM might predict the risk of relapse and death, with potential implications for determining which subgroups of UMs are amenable to specific pharmacological treatment regimens.
尽管葡萄膜黑色素瘤（UM）主要发生在白种人人群中，但它是成年人中最常见的原发性眼内肿瘤。在此，我们使用数字病理学和图像分析来诊断UM并预测预后。我们的回顾性研究共纳入了来自101例患者的404张组织病理学切片。对组织免疫生物标志物（CD4、CD8、CD68、CD163）进行了数字图像采集和定量分析。检测到肿瘤内CD8阳性细胞密度高于13.3个细胞/mm对无复发生存期（RFS，风险比[HR] 2.08，95%置信区间[Cl] 1.09至3.99，P = 0.027）和总生存期（OS，HR 3.30，95% CI 1.58至6.88，P = 0.001）均有负面影响。此外，我们证实年龄较大和III期是RFS和OS的独立负面预后因素。我们的结果表明，UM中CD8的特定分布模式可能预测复发和死亡风险，这对于确定哪些UM亚组适合特定的药物治疗方案具有潜在意义。
