Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Technical Biochemistry, Department of Biochemical and Chemical Engineering, Technical University of Dortmund, 44227 Dortmund, Germany.
Molecules. 2022 Nov 24;27(23):8189. doi: 10.3390/molecules27238189.
Jacq. (Solanaceae) is widely found in South East Asia. In Thailand, it is used as vegetable and as a component in traditional recipes. The results of an alpha-glucosidase inhibitory screening test found that the crude extract of inflorescence exhibited the potential effect with IC 81.27 μg/mL. The separation was performed by the increasing solvent polarity method. The ethyl acetate, ethanol, and water extracts of inflorescence showed the synergistic effect together with acarbose standard. The phytochemical investigation of these extracts was conducted by chromatographic and spectroscopic techniques. Six flavonoid compounds, myricetin 3, 4', 5', 7-tetramethyl ether (), combretol (), kaempferol (), kaempferol 7--glucopyranoside (), 5-hydroxy 3-7-4'-5'-tetramethoxyflavone-3'--glucopyranoside (), and a mixture () of isorhamnetin 3--glucopyranoside () and astragalin () were isolated. This discovery is the first report of flavonoid-glycoside . Moreover, the selected flavonoids, kaempferol and astragalin, were representatives to explore the mechanism of action. Both of them performed mixed-type inhibition. The molecular docking gave a better understanding of flavonoid compounds' ability to inhibit the alpha-glucosidase enzyme.
莕菜属（Solanaceae）广泛分布于东南亚。在泰国，它被用作蔬菜和传统食谱的成分。α-葡萄糖苷酶抑制筛选试验的结果表明，花序的粗提取物具有潜在的效果，IC 81.27 μg/mL。分离采用增加溶剂极性的方法。莕菜属花序的乙酸乙酯、乙醇和水提取物与阿卡波糖标准品表现出协同作用。通过色谱和光谱技术对这些提取物进行了植物化学成分研究。从这些提取物中分离得到了 6 种黄酮类化合物，杨梅素 3,4',5',7-四甲基醚（）、考布醇（）、山柰酚（）、山柰酚 7--吡喃葡萄糖苷（）、5-羟基 3-7-4'-5'-四甲氧基黄酮-3'--吡喃葡萄糖苷（）和异鼠李素 3--吡喃葡萄糖苷（）和槲皮素（）的混合物（）。这一发现是首次报道黄酮苷。此外，选择的黄酮类化合物山奈酚和槲皮素被用来探索它们的作用机制。它们都表现出混合抑制作用。分子对接更好地理解了黄酮类化合物抑制α-葡萄糖苷酶的能力。
