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植物合成生物学:从灵感到增强。

Plant synthetic biology: from inspiration to augmentation.

机构信息

Metabolite Diversity Group, Department of Molecular Genetics, Leibniz Institute for Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Correnstr. 3, D-06466 Seeland, Germany.

Metabolite Diversity Group, Department of Molecular Genetics, Leibniz Institute for Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Correnstr. 3, D-06466 Seeland, Germany.

出版信息

Curr Opin Biotechnol. 2023 Feb;79:102857. doi: 10.1016/j.copbio.2022.102857. Epub 2022 Dec 8.

Abstract

Although it is still in its infancy, synthetic biology has the capacity to face scientific and societal problems related to modern agriculture. Innovations in cloning toolkits and genetic parts allow increased precision over gene expression in planta. We review the vast spectrum of available technologies providing a practical list of toolkits that take advantage of combinatorial power to introduce/alter metabolic pathways. We highlight that rational design is inspired by deep knowledge of natural and biochemical mechanisms. Finally, we provide several examples in which modern technologies have been applied to address these critical topics. Future applications in plants include not only pathway modifications but also prospects of augmenting plant anatomical features and developmental processes.

摘要

尽管合成生物学仍处于起步阶段,但它有能力应对与现代农业相关的科学和社会问题。克隆工具包和遗传元件的创新使得在植物体内实现基因表达的精度得到提高。我们回顾了广泛的可用技术,提供了一个实用的工具包列表,这些工具包利用组合能力来引入/改变代谢途径。我们强调,理性设计是受到对自然和生化机制的深刻理解的启发。最后,我们提供了几个应用现代技术来解决这些关键问题的例子。未来在植物中的应用不仅包括途径修饰,还包括增强植物解剖特征和发育过程的前景。

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