Yao Xinghao, Taheri Ayat, Liu Hang, Zhang Yaojie, Li Ling, Shao Jin, Wu Ke, Miao Qing, He Weizhi, Hu Xinyi, Tang Kexuan
Joint International Research Laboratory of Metabolic and Developmental Sciences, Frontiers Science Center for Transformative Molecules, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Hortic Res. 2024 Jul 26;11(9):uhae197. doi: 10.1093/hr/uhae197. eCollection 2024 Sep.
The -mediated transient expression system has been developed and applied to various plants as an alternative to stable transformation. However, its application in tomatoes is still limited due to low expression efficiency. In this study, we describe an improved vacuum-infiltration system that can be used in both tomato fruits and leaves. Notably, this study is the first report of vacuum infiltration in attached tomato fruits. The feasibility of the improved vacuum-infiltration system in Micro-Tom tomato was confirmed by various assays, including multiple fluorescent protein expression analysis, -glucuronidase activity analysis, and reporter visualization. Subsequently, the improved vacuum-infiltration system was successfully applied to tomato biotechnology research. Herein, a trichome-specific promoter in tomato was identified that can drive the directional synthesis of specific plant natural products (PNPs). Additionally, based on the assessment results of the improved vacuum-infiltration system, we obtained a flavonoid-rich tomato variety through the stable transformation of and . In a significant practical application, we successfully synthesized the high-value scutellarin in tomato, which provides an alternative route for the production of PNPs from plants. In addition, the improved vacuum-infiltration system has been demonstrated to be suitable for commercial tomato varieties ('Emerald' and 'Provence') as well. The improved vacuum-infiltration system not only speeds up fundamental and applied research in tomato but also offers an additional powerful tool for advancing tomato synthetic biology research.
介导的瞬时表达系统已被开发并应用于各种植物，作为稳定转化的替代方法。然而，由于表达效率低，其在番茄中的应用仍然有限。在本研究中，我们描述了一种改进的真空渗透系统，该系统可用于番茄果实和叶片。值得注意的是，本研究是关于附着番茄果实真空渗透的首次报道。通过多种检测方法，包括多种荧光蛋白表达分析、β-葡萄糖醛酸酶活性分析和报告基因可视化，证实了改进的真空渗透系统在微型番茄中的可行性。随后，改进的真空渗透系统成功应用于番茄生物技术研究。在此，鉴定了番茄中的一种毛状体特异性启动子，其可驱动特定植物天然产物（PNP）的定向合成。此外，基于改进的真空渗透系统的评估结果，我们通过对……和……的稳定转化获得了一个富含类黄酮的番茄品种。在一项重要的实际应用中，我们成功地在番茄中合成了高价值的灯盏花素，这为从植物中生产PNP提供了一条替代途径。此外，改进的真空渗透系统已被证明也适用于商业番茄品种（“翡翠”和“普罗旺斯”）。改进的真空渗透系统不仅加快了番茄的基础研究和应用研究，还为推进番茄合成生物学研究提供了另一个强大工具。
