Chacón Micaela, Dixon Neil
Manchester Institute of Biotechnology (MIB), Department of Chemistry, University of Manchester, Manchester, M1 7DN, UK.
Metab Eng Commun. 2024 Nov 28;19:e00255. doi: 10.1016/j.mec.2024.e00255. eCollection 2024 Dec.
Current plastic production and consumption routes are unsustainable due to impact upon climate change and pollution, and therefore reform across the entire value chain is required. Biotechnology offers solutions for production from renewable feedstocks, and to aid end of life recycling/upcycling of plastics. Biology sequence/design space is complex requiring high-throughput analytical methods to facilitate the iterative optimisation, design-build, test-learn (DBTL), cycle of Synthetic Biology. Furthermore, genetic regulatory tools can enable harmonisation between biotechnological demands and the physiological constraints of the selected production host. Genetically encoded biosensors offer a solution for both requirements to facilitate the circular plastic bioeconomy. In this review we present a summary of biosensors developed to date reported to be responsive to plastic precursors/monomers. In addition, we provide a summary of the demonstrated and prospective applications of these biosensors for the construction and deconstruction of plastics. Collectively, this review provides a valuable resource of biosensor tools and enabled applications to support the development of the circular plastics bioeconomy.
由于对气候变化和污染产生影响，当前的塑料生产和消费途径是不可持续的，因此需要对整个价值链进行改革。生物技术为利用可再生原料进行生产以及助力塑料的生命周期末期回收/升级回收提供了解决方案。生物序列/设计空间很复杂，需要高通量分析方法来促进合成生物学的迭代优化、设计-构建-测试-学习（DBTL）循环。此外，基因调控工具能够使生物技术需求与所选生产宿主的生理限制相协调。基因编码生物传感器为满足这两个要求提供了一种解决方案，以促进循环塑料生物经济的发展。在本综述中，我们总结了迄今为止已报道的对塑料前体/单体有响应的生物传感器。此外，我们还总结了这些生物传感器在塑料的构建和解构方面已证明的和潜在的应用。总体而言，本综述提供了有价值的生物传感器工具及相关应用资源，以支持循环塑料生物经济的发展。
