Wang Xiaoxia, Ren Xiao-Min, He Huan, Li Fan, Liu Kunqian, Zhao Fenqing, Hu Huixiang, Zhang Pingping, Huang Bin, Pan Xuejun
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Toxicology. 2023 Jan 15;484:153391. doi: 10.1016/j.tox.2022.153391. Epub 2022 Dec 9.
Numerous studies have shown that exposure to micro- or nano-plastics led to the cell viability and function of macrophages in the intestine tissue might be one possible mechanism. This study investigated the cytotoxicity and pro-inflammatory effect of 80 nm polystyrene-nano-plastic (PS-NP) and 3 µm PS-micro-plastic (PS-MP) on mouse macrophages RAW264.7 cells. Our results showed that exposure to PS-NP or PS-MP induced apoptosis of cells at 5 or 10 μg/mL, respectively. Besides, PS-NP enhanced the secretion of inflammatory cytokines (Tumor necrosis factor-α, Interleukin-6 and Interleukin-10) with the lowest effective concentration (LOEC) of 1, 0.01, and 0.01 μg/mL, respectively. PS-MP enhanced secretion of TNF-α and IL-10 with the LOEC of 1 and 0.01 μg/mL, respectively. We further studied the possible mechanisms of the effects of PS-NP or PS-MP on RAW264.7 cells. We found they might cause cytotoxicity and inflammatory effects by producing reactive oxygen species and nitric oxide in the cells. Accordingly, our results demonstrated that PS-NP and PS-MP had cytotoxicity and pro-inflammatory effect on macrophages, which might further lead to intestinal inflammation. Moreover, we revealed that the PS-NP had more potent adverse impacts on macrophages than PS-MP.
众多研究表明，接触微塑料或纳米塑料导致肠道组织中巨噬细胞的细胞活力和功能受到影响可能是一种潜在机制。本研究调查了80纳米聚苯乙烯纳米塑料（PS-NP）和3微米聚苯乙烯微塑料（PS-MP）对小鼠巨噬细胞RAW264.7细胞的细胞毒性和促炎作用。我们的结果显示，分别以5微克/毫升或10微克/毫升的浓度接触PS-NP或PS-MP会诱导细胞凋亡。此外，PS-NP分别以1微克/毫升、0.01微克/毫升和0.01微克/毫升的最低有效浓度增强了炎性细胞因子（肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10）的分泌。PS-MP分别以1微克/毫升和0.01微克/毫升的最低有效浓度增强了肿瘤坏死因子-α和白细胞介素-10的分泌。我们进一步研究了PS-NP或PS-MP对RAW264.7细胞产生影响的可能机制。我们发现它们可能通过在细胞中产生活性氧和一氧化氮而导致细胞毒性和炎症效应。因此，我们的结果表明，PS-NP和PS-MP对巨噬细胞具有细胞毒性和促炎作用，这可能会进一步导致肠道炎症。此外，我们发现PS-NP对巨噬细胞的不利影响比PS-MP更强。
