Laboratory Reig Jofre, R&D Department, 08970Sant Joan Despi, Barcelona, Spain.
Molecular Pharmacology and Experimental Therapeutics, Department of Biochemistry and Molecular. Biomedicine, Institute of Biomedicine (IBUB), University of Barcelona (IBUB), 08028Barcelona, Spain.
Mol Pharm. 2023 May 1;20(5):2317-2325. doi: 10.1021/acs.molpharmaceut.2c00796. Epub 2022 Dec 12.
Latanoprost (LAT) has been shown to have a hypertrichotic effect, which makes it a promising candidate for alopecia treatments. For the first time, LAT has been encapsulated in nanotransfersomes in order to increase its efficacy. skin biodistribution was studied by confocal laser microscopy both in human scalp and pig skin. Results showed that nanotransfersomes increase the penetration of two different fluorochromes, with similar patterns in both species, compared with fluorochrome solutions containing no nanotransfersomes. Nanotransfersomes were stable under accelerated conditions (40 °C/75% RH) and long-term conditions (25 °C/60% RH) for up to 1 year, with no differences in vesicle size and polydispersity when LAT was loaded. Nanotransfersomes increased the LAT cell proliferation effect in HaCaT cell via MAPK signaling pathway. Collectively, our results demonstrate LAT-nanotransfersomes formulation could be a promising therapy for hair growth disorders.
拉坦前列素 (LAT) 已被证明具有多毛作用，使其成为治疗脱发的有前途的候选药物。 拉坦前列素首次被包封在纳米转体中以提高其功效。 通过共聚焦激光显微镜研究了纳米转体在人头皮和猪皮中的皮肤分布。结果表明，与不含纳米转体的荧光染料溶液相比，纳米转体增加了两种不同荧光染料的穿透性，两种物种的穿透模式相似。纳米转体在加速条件（40°C/75% RH）和长期条件（25°C/60% RH）下稳定长达 1 年，当负载拉坦前列素时，囊泡大小和多分散性没有差异。纳米转体通过 MAPK 信号通路增加了 HaCaT 细胞中 LAT 的细胞增殖作用。综上所述，我们的结果表明 LAT-纳米转体制剂可能是治疗毛发疾病的有前途的疗法。
