Li Shiling, Sun Shuhan, Qi Fei, Dou Xiaomin
Beijing Key Lab for Source Control Technology of Water Pollution, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.
Engineering Research Center for Water Pollution Source Control and Eco-remediation, College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China.
J Environ Sci (China). 2023 Apr;126:287-296. doi: 10.1016/j.jes.2022.05.027. Epub 2022 May 27.
Antibiotic production wastewater usually contains high concentrations of antibiotic residues, which can cause instability and deterioration of biological wastewater treatment units and also domestication and proliferation of antibiotic-resistance bacteria. An effective pretreatment on antibiotics production wastewater is expected to selectively reduce the concentration of antibiotics and decrease the toxicity, rather than mitigate organic and other contaminants before further treatments. In this work, two polymer-based solid acids, PS-S and CPS-S bearing high concentrations of -SOH groups (up to 4.57 mmol/g), were prepared and successfully used for hydrolytic mitigation of 100 mg/L tylosin within 20 min. The co-existence of high concentrations of COD and humic substances did not affect the mitigation of tylosin obviously, while more than 500 mg/L of nitrogenous compounds suppressed the hydrolytic efficiency. Recycle and reuse experiments showed that the solid acids performed well in five cycles after regeneration. Three transformation products (P1, P2 and P3) were identified using UPLC-QTOF-MS/MS. Sugar moieties including mycarse, mycaminose, and mycinose detached and released simultaneously or in order from the 16-member lactone ring through desugarization, which led to a dramatic decrease in antibacterial activity as revealed by cytotoxicity evaluations using S. aureus. Ecotoxicity estimation indicated the acute toxicities of the hydrolyzed products to model species (e.g., fish, daphnid and green algae) were classified as "not harmful". This work suggested an effective and selective method to pretreat tylosin-contained production wastewater by using polymer-based solid acids. These results will shed light on effective elimination of antibiotics pollution from pharmaceutical industries through strengthening the pretreatments.
抗生素生产废水通常含有高浓度的抗生素残留，这可能导致生物废水处理单元的不稳定和恶化，还会引起抗生素抗性细菌的驯化和增殖。预计对抗生素生产废水进行有效的预处理可以选择性地降低抗生素浓度并降低毒性，而不是在进一步处理之前减轻有机和其他污染物。在这项工作中，制备了两种基于聚合物的固体酸PS-S和CPS-S，它们含有高浓度的-SOH基团（高达4.57 mmol/g），并成功用于在20分钟内对100 mg/L泰乐菌素进行水解降解。高浓度的化学需氧量（COD）和腐殖物质的共存并没有明显影响泰乐菌素的降解，而超过500 mg/L的含氮化合物会抑制水解效率。循环和再利用实验表明，固体酸在再生后的五个循环中表现良好。使用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）鉴定了三种转化产物（P1、P2和P3）。包括霉糖、霉胺糖和霉糖在内的糖部分通过脱糖作用同时或依次从16元内酯环上脱离并释放出来，这导致金黄色葡萄球菌细胞毒性评估显示抗菌活性急剧下降。生态毒性评估表明，水解产物对模式生物（如鱼类、水蚤和绿藻）的急性毒性被归类为“无害”。这项工作提出了一种使用基于聚合物的固体酸预处理含泰乐菌素生产废水的有效且选择性的方法。这些结果将为通过加强预处理有效消除制药行业的抗生素污染提供启示。
