Department of Thoracic Surgery, National Clinical Research Center for Infectious Diseases, Third People's Hospital of Shenzhen, Shenzhen, Guangdong, China.
Clinical Laboratory, National Clinical Research Center for Infectious Diseases, Third People's Hospital of Shenzhen, Shenzhen, Guangdong, China.
Biosci Trends. 2022 Dec 26;16(6):451-454. doi: 10.5582/bst.2022.01498. Epub 2022 Dec 9.
A saline gargle (SG) has proven to be an efficient method of sampling to detect SARS-CoV-2. The aim of this pilot study was to verify the efficiency of SG sampling in detecting the Omicron variant of SARS-CoV-2. Subjects were a total of 68 patients with COVID-19 (Omicron variant), and 167 pairs of samples were collected. A conventional oropharyngeal swab (OPS) was obtained and SG sampling was performed immediately afterward; both were subjected to RT-qPCR. A subgroup analysis of symptomatic and asymptomatic patients was performed. Results revealed no significant differences in the distribution of patients and cycle threshold (CT) values between the SG and OPS in overall data and data on days 1-3, 4-7, and 8-14. The subgroup analysis revealed no significant differences between the SG and OPS results in symptomatic patients. In asymptomatic patients, the CT values for the SG were significantly lower than those for the OPS, implying that SG sampling had better sensitivity in the context of the Omicron variant. These data indicate that the SG had satisfactory efficiency (vs. the OPS). An SG is a simple and less invasive method of sampling that is suited to mass, frequent, and repeated sampling to detect SARS-CoV-2.
含盐水漱口（SG）已被证明是一种有效的采样方法，可用于检测 SARS-CoV-2。本初步研究旨在验证 SG 采样检测 SARS-CoV-2 的奥密克戎变异株的效率。研究对象为总共 68 名 COVID-19（奥密克戎变异株）患者，共采集了 167 对样本。采集了常规的咽拭子（OPS）和 SG 样本，并立即对这两种样本进行 RT-qPCR 检测。对有症状和无症状患者进行了亚组分析。结果显示，在总体数据和第 1-3 天、第 4-7 天和第 8-14 天的数据中，SG 和 OPS 的患者分布和循环阈值（CT）值均无显著差异。亚组分析显示，在有症状患者中，SG 和 OPS 的检测结果无显著差异。在无症状患者中，SG 的 CT 值明显低于 OPS，这意味着在奥密克戎变异株的情况下，SG 采样具有更好的灵敏度。这些数据表明 SG 的效率令人满意（与 OPS 相比）。SG 是一种简单、微创的采样方法，适合用于大规模、频繁、重复的采样，以检测 SARS-CoV-2。
