Xu Peng, Chen Jing, Qian Chengchen, Yu Wenqiang
Laboratory of RNA Epigenetics, Institutes of Biomedical Sciences & Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai, China.
Research and Development Department, Shanghai Epiprobe Biotechnology Co., Ltd, Shanghai, China.
Biosci Trends. 2023 Mar 11;17(1):81-84. doi: 10.5582/bst.2022.01530. Epub 2023 Jan 17.
A self‑collected gargle sample, which avoids discomfort and largely reduces the dependency on medical resources, is emerging for detection of SARS‑CoV‑2. However, the incomplete usage of starting materials for both routine oropharyngeal swabs (OPS)/nasopharyngeal swabs (NPS) and saline gargle (SG) samples implies sensitivity can be further improved. Presented here is a bead‑based strategy for pre‑enrichment of SG samples, and results revealed that it acquired about 20 times the starting materials obtained from OPS samples for downstream detection of SARS‑CoV‑2. The sensitivity and specificity of this pre‑enrichment strategy were validated in 100 paired pre‑enriched saline gargle (PenSG) and OPS samples and 89 PenSG samples from healthy volunteers. In addition to detection of SARS‑CoV‑2, this pre‑enrichment strategy may also be implemented in more clinical settings to optimize detection of other diseases.
一种自行采集的漱口液样本正在用于检测新型冠状病毒（SARS-CoV-2），这种样本避免了不适感，并在很大程度上减少了对医疗资源的依赖。然而，常规口咽拭子（OPS）/鼻咽拭子（NPS）和盐水漱口液（SG）样本起始材料的使用不充分意味着灵敏度可以进一步提高。本文介绍了一种基于磁珠的策略用于SG样本的预富集，结果显示，在下游检测SARS-CoV-2时，该策略获得的起始材料约为OPS样本的20倍。这种预富集策略的灵敏度和特异性在100对预富集盐水漱口液（PenSG）和OPS样本以及89份来自健康志愿者的PenSG样本中得到了验证。除了检测SARS-CoV-2外，这种预富集策略还可以在更多临床环境中实施，以优化其他疾病的检测。
