Wan Yongjie, Li Dongxu, Deng Mingtian, Liu Zifei, Liu Liang, Wang Feng
Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Biology (Basel). 2021 Apr 28;10(5):381. doi: 10.3390/biology10050381.
It has been reported that hypoxic environments were more suitable for the in vitro development of mammalian embryos, but the underlying mechanisms were still unclear. In the present study, RNA-seq was performed to compare 8-cell-stage and blastocyst-stage goat embryos under hypoxic and normoxic conditions; zygotes were checked at 72 and 168 h to 8-cell stage (L8C) and blastocyst stage (LM) in hypoxic conditions and 8-cell stage (H8C) and blastocyst stage (HM) in normoxic conditions. In the H8C and L8C groups, 399 DEGs were identified, including 348 up- and 51 down-regulated DEGs. In the HM and LM groups, 1710 DEGs were identified, including 1516 up- and 194 down-regulated DEGs. The expression levels of zygotic genes, transcription factors, and maternal genes, such as and showed significant changes. Functional enrichment analysis indicated that these DEGs were mainly related to biological processes and function regulation. In addition, combined with the pathway-gene interaction network and protein-protein interaction network, twenty-two of the hub genes were identified and they are mainly involved in energy metabolism, immune stress response, cell cycle, receptor binding, and signal transduction pathways. The present study provides comprehensive insights into the effects of oxidative stress on early embryo development in goats.
据报道，低氧环境更适合哺乳动物胚胎的体外发育，但其潜在机制仍不清楚。在本研究中，进行了RNA测序，以比较低氧和常氧条件下8细胞期和囊胚期的山羊胚胎；合子在低氧条件下于72小时和168小时检查至8细胞期（L8C）和囊胚期（LM），在常氧条件下检查至8细胞期（H8C）和囊胚期（HM）。在H8C和L8C组中，鉴定出399个差异表达基因（DEG），包括348个上调和51个下调的DEG。在HM和LM组中，鉴定出1710个DEG，包括1516个上调和194个下调的DEG。合子基因、转录因子和母体基因（如 和 ）的表达水平发生了显著变化。功能富集分析表明，这些DEG主要与生物学过程和功能调节有关。此外，结合通路-基因相互作用网络和蛋白质-蛋白质相互作用网络，鉴定出22个枢纽基因，它们主要参与能量代谢、免疫应激反应、细胞周期、受体结合和信号转导通路。本研究为氧化应激对山羊早期胚胎发育的影响提供了全面的见解。
