Hernandez-Lima Mirella A, Champion Margaret, Mattiola Zachary, Truttmann Matthias C
Neuroscience Graduate Program, University of Michigan, Ann Arbor, MI, United States.
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, United States.
Front Mol Neurosci. 2022 Nov 24;15:912734. doi: 10.3389/fnmol.2022.912734. eCollection 2022.
Post-translational protein modifications are essential for the spatio-temporal regulation of protein function. In this study, we examine how the activity of the AMPylase FIC-1 modulates physiological processes . We find that over-expression (OE) of the constitutive AMPylase FIC-1(E274G) impairs development, fertility, and stress resilience. We also show that FIC-1(E274G) OE inhibits pathogen avoidance behavior by selectively suppressing production of the Transforming Growth Factor-β (TGF-β) ligands DAF-7 and DBL-1 in ASI sensory neurons. Finally, we demonstrate that FIC-1 contributes to the regulation of adult body growth, cholinergic neuron function, and larval entry into dauer stage; all processes controlled by TGF-β signaling. Together, our results suggest a role for FIC-1 in regulating TGF-β signaling in .
蛋白质翻译后修饰对于蛋白质功能的时空调节至关重要。在本研究中，我们研究了AMPylase FIC-1的活性如何调节生理过程。我们发现组成型AMPylase FIC-1(E274G)的过表达会损害发育、生育能力和应激恢复力。我们还表明，FIC-1(E274G)的过表达通过选择性抑制ASI感觉神经元中转化生长因子-β (TGF-β)配体DAF-7和DBL-1的产生来抑制病原体回避行为。最后，我们证明FIC-1有助于调节成虫身体生长、胆碱能神经元功能以及幼虫进入滞育期；所有这些过程都受TGF-β信号传导控制。总之，我们的结果表明FIC-1在调节TGF-β信号传导中发挥作用。
