用于活细胞中蛋白 AMP 化成像的 Pronucleotide 探针。
A Pronucleotide Probe for Live-Cell Imaging of Protein AMPylation.
机构信息
Department of Chemistry, Technical University of Munich, Lichtenbergstrasse 4, 85748, Garching, Germany.
Max Planck Institute of Psychiatry, Kraepelinstrasse 2, 80804, Munich, Germany.
出版信息
Chembiochem. 2020 May 4;21(9):1285-1287. doi: 10.1002/cbic.201900716. Epub 2020 Feb 6.
Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
蛋白质与 AMP 的结合（AMPylation）是人类细胞中一种普遍的翻译后修饰（PTM），参与未折叠蛋白反应和神经发育的调节。在这里，我们提出了一种定制的前核苷探针，适用于 AMP 化蛋白质的原位成像和化学蛋白质组学分析。该探针通过简单的应变促进叠氮化物-炔烃点击化学，在活细胞中提供稳定的荧光标记。
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