Li Tong, Wei Yongjun, Zhao Chenchen, Li Shaojian, Gao Suxia, Zhang Yuanchen, Wu Yuqing, Lu Chuantao
Institute of Plant Protection, Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, Zhengzhou, China.
School of Pharmaceutical Sciences, Laboratory of Synthetic Biology, Zhengzhou University, Zhengzhou, China.
Front Microbiol. 2022 Nov 23;13:1020461. doi: 10.3389/fmicb.2022.1020461. eCollection 2022.
Aphids are major crop pests, and they can be controlled through the application of the promising RNA interference (RNAi) techniques. However, chemical synthesis yield of dsRNA for RNAi is low and costly. Another sustainable aphid pest control strategy takes advantage of symbiont-mediated RNAi (SMR), which can generate dsRNA by engineered microbes. Aphid host the obligate endosymbiont and various facultative symbionts that not only have a wide host range but are also vertically and horizontally transmitted. Thus, we described the potential of facultative symbionts in aphid pest control by SMR. We summarized the community and host range of these facultative symbionts, and then reviewed their probable horizontal transmitted routes and ecological functions. Moreover, recent advances in the cultivation and genetic engineering of aphid facultative symbionts were discussed. In addition, current legislation of dsRNA-based pest control strategies and their safety assessments were reviewed.
蚜虫是主要的农作物害虫，可以通过应用有前景的RNA干扰（RNAi）技术来进行防治。然而，用于RNAi的dsRNA的化学合成产量低且成本高。另一种可持续的蚜虫害虫防治策略利用共生菌介导的RNA干扰（SMR），它可以通过工程微生物产生dsRNA。蚜虫宿主专性内共生菌和各种兼性共生菌，这些共生菌不仅宿主范围广泛，而且可以垂直和水平传播。因此，我们描述了兼性共生菌在通过SMR防治蚜虫害虫方面的潜力。我们总结了这些兼性共生菌的群落和宿主范围，然后回顾了它们可能的水平传播途径和生态功能。此外，还讨论了蚜虫兼性共生菌培养和基因工程的最新进展。此外，还综述了基于dsRNA的害虫防治策略的现行法规及其安全性评估。
