National Health Laboratory Service, DST/NRF Centre of Excellence for Biomedical TB Research, University of the Witwatersrand, Johannesburg, South Africa.
Perinatal HIV Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Front Cell Infect Microbiol. 2022 Nov 24;12:1072073. doi: 10.3389/fcimb.2022.1072073. eCollection 2022.
Tuberculosis (TB) infected individuals harbor a heterogenous population of differentially culturable tubercle bacilli (DCTB). Herein, we describe how DCTB assays using culture filtrate either containing or deficient in resuscitation promoting factors can uncover mixed infections. We demonstrate that () strain genotypes can be separated in DCTB assays based on their selective requirement for growth stimulatory factors. Beijing mixed infections appear to be associated with a higher bacterial load and reduced reliance on growth stimulatory factors. These data have important implications for identifying mixed infections and hetero-resistance, which in turn can affect selection of treatment regimen and establishment of transmission links.
结核分枝杆菌（TB）感染者体内存在异质性的可培养分枝杆菌（DCTB）群体。在此，我们描述了如何使用含有或缺乏复苏促进因子的培养滤液来进行 DCTB 检测，从而发现混合感染。我们证明，根据其对生长刺激因子的选择性需求，（）株基因型可以在 DCTB 检测中分离。北京混合感染似乎与更高的细菌负荷和对生长刺激因子的依赖减少有关。这些数据对于识别混合感染和异质性耐药具有重要意义，这反过来又会影响治疗方案的选择和传播途径的建立。
