State Key Laboratory of Advanced Electromagnetic Engineering and Technology, School of Electrical and Electronic Engineering, Huazhong University of Science and Technology, 430074 Wuhan, China.
The GBA National Institute for Nanotechnology Innovation, China.
Oxid Med Cell Longev. 2022 Nov 30;2022:1838202. doi: 10.1155/2022/1838202. eCollection 2022.
Infected wound is one of the most common and serious problem in wound management. Cold atmospheric plasma (CAP) is considered to have a good effect in wound healing as a new type medicine. However, there is a key issue that has not been addressed in the treatment of infected wounds by plasma. Bacteria are always found in the deep region of the wound. When plasma is used to treat wounds, it also acts on normal tissue cells while decontaminating. What is the difference between the same dose of plasma acting on bacteria and normal cells? In this study, the most common bacteria (S. aureus, P. aeruginosa, and E. coli) in infected wound and two kinds of normal skin cells (human keratinocyte and human skin fibroblasts (HSF)) were selected to study the difference of the effects of the same dose of plasma on bacteria and cells. The results reveal that three kinds of 10 CFU mL bacteria could be effectively inactivated by 5 order after plasma treatment 3 min, and P. aeruginosa was more sensitive to plasma (could be inactivated 5 order after 2 min treatment). The 10 mL keratinocyte and HSF were treated with the same dose of plasma; keratinocyte can maintain over 90% of the activity and HSF cells can maintain over 70% of the activity. Moreover, the level of collagen I secreted by HSF increased. Therefore, cells can remain a high activity when a plasma dose capable of inactivating bacteria is applied to them.
感染性伤口是伤口管理中最常见和最严重的问题之一。冷等离子体（CAP）被认为是一种新型药物，在伤口愈合方面有很好的效果。然而，在等离子体治疗感染性伤口时，有一个关键问题尚未得到解决。细菌总是存在于伤口的深部。当等离子体用于治疗伤口时，它在消毒的同时也作用于正常组织细胞。相同剂量的等离子体作用于细菌和正常细胞有什么区别？在这项研究中，选择了感染性伤口中最常见的三种细菌（金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌）和两种正常皮肤细胞（人角质形成细胞和人皮肤成纤维细胞（HSF））来研究相同剂量等离子体对细菌和细胞的影响的差异。结果表明，三种 10 CFU/mL 的细菌经等离子体处理 3 分钟后可有效失活 5 个数量级，铜绿假单胞菌对等离子体更敏感（处理 2 分钟后可失活 5 个数量级）。用相同剂量的等离子体处理 10 mL 角质形成细胞和 HSF；角质形成细胞的活性可以保持在 90%以上，HSF 细胞的活性可以保持在 70%以上。此外，HSF 分泌的胶原蛋白 I 水平增加。因此，当应用能够使细菌失活的等离子体剂量时，细胞可以保持高活性。
