Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
Anim Sci J. 2022 Jan-Dec;93(1):e13787. doi: 10.1111/asj.13787.
Uterine collagen is the most abundant component of the uterine extracellular matrix and plays a critical role in pregnancy. The 47-kDa heat shock protein (HSP47) is the sole collagen-specific molecular chaperone. We investigated the mechanisms regulating the expression of HSP47 in the uterus by assessing the effect of anti-Müllerian hormone (AMH) stimulation on HSP47 expression in cultured bovine uterine epithelial cells. AMH receptor type 2 (AMHR2), AMH, and HSP47 expression was assessed by fluorescence immunocytochemistry in uterine epithelial layers of the uteri of Japanese Black cows. The effect of AMH on HSP47 expression was assessed in cultured epithelial cells. The effect of MEK/ERK inhibitor on AMH-induced HSP47 expression was also assessed. We confirmed the expression of AMHR2, AMH, and HSP47 in the uterine epithelial layers. We confirmed the expression of AMHR2, AMH, HSP47, and type IV collagen in cultured uterine epithelial cells. AMH treatment at 10 or 100 ng/ml promoted significant HSP47 expression (p < 0.05). MEK/ERK inhibitor U0126 pretreatment suppressed such AMH stimulation on HSP47. These findings indicate that AMH induced HSP47 protein expression through the ERK pathway in bovine uterine epithelial cells.
子宫胶原是子宫细胞外基质中最丰富的成分，在妊娠中起着关键作用。47kDa 热休克蛋白（HSP47）是唯一的胶原特异性分子伴侣。我们通过评估抗苗勒管激素（AMH）刺激对培养的牛子宫上皮细胞中 HSP47 表达的影响，研究了调节 HSP47 在子宫中表达的机制。通过荧光免疫细胞化学评估日本黑牛子宫上皮层中 AMH 受体 2（AMHR2）、AMH 和 HSP47 的表达。评估 AMH 对 HSP47 表达的影响。还评估了 MEK/ERK 抑制剂对 AMH 诱导的 HSP47 表达的影响。我们证实了 AMHR2、AMH 和 HSP47 在子宫上皮层中的表达。我们证实了 AMHR2、AMH、HSP47 和 IV 型胶原在培养的子宫上皮细胞中的表达。10 或 100ng/ml 的 AMH 处理促进了 HSP47 的显著表达（p<0.05）。MEK/ERK 抑制剂 U0126 预处理抑制了 AMH 对 HSP47 的这种刺激。这些发现表明，AMH 通过牛子宫上皮细胞中的 ERK 途径诱导 HSP47 蛋白表达。
