通过鼻内接种后神经元侵犯导致视网膜炎症的动物模型中，SARS-CoV-2 的眼部趋向性。

Ocular tropism of SARS-CoV-2 in animal models with retinal inflammation via neuronal invasion following intranasal inoculation.

机构信息

Department of Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, 34114, Republic of Korea.

Department of Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.

出版信息

Nat Commun. 2022 Dec 12;13(1):7675. doi: 10.1038/s41467-022-35225-1.

DOI:10.1038/s41467-022-35225-1

PMID:36509737

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9743116/

Abstract

Although ocular manifestations are reported in patients with COVID-19, consensus on ocular tropism of SARS-CoV-2 is lacking. Here, we infect K18-hACE2 transgenic mice with SARS-CoV-2 using various routes. We observe ocular manifestation and retinal inflammation with production of pro-inflammatory cytokines in the eyes of intranasally (IN)-infected mice. Intratracheal (IT) infection results in dissemination of the virus from the lungs to the brain and eyes via trigeminal and optic nerves. Ocular and neuronal invasions are confirmed using intracerebral (IC) infection. Notably, the eye-dropped (ED) virus does not cause lung infection and becomes undetectable with time. Ocular and neurotropic distribution of the virus in vivo is evident in fluorescence imaging with an infectious clone of SARS-CoV-2-mCherry. The ocular tropic and neuroinvasive characteristics of SARS-CoV-2 are confirmed in wild-type Syrian hamsters. Our data can improve the understanding regarding viral transmission and clinical characteristics of SARS-CoV-2 and help in improving COVID-19 control procedures.

摘要

虽然有报道称 COVID-19 患者存在眼部表现，但对 SARS-CoV-2 的眼向性仍缺乏共识。在这里，我们使用各种途径感染 K18-hACE2 转基因小鼠 SARS-CoV-2。我们观察到经鼻腔（IN）感染的小鼠出现眼部表现和视网膜炎症，并在眼睛中产生促炎细胞因子。通过三叉神经和视神经，气管内（IT）感染导致病毒从肺部传播到大脑和眼睛。通过脑内（IC）感染证实了眼部和神经元入侵。值得注意的是，滴眼（ED）病毒不会引起肺部感染，并且随着时间的推移变得无法检测。使用 SARS-CoV-2-mCherry 的感染性克隆进行荧光成像，显示了病毒在体内的眼向性和神经侵袭性分布。在野生型叙利亚仓鼠中证实了 SARS-CoV-2 的眼向性和神经侵袭性特征。我们的数据可以提高对 SARS-CoV-2 病毒传播和临床特征的认识，并有助于改进 COVID-19 控制程序。