Research Institute of Therapy and Preventive Medicine, Affiliation of the Federal Research Center Institute of Cytology and Genetics.
Meshalkin National Medical Research Center.
Kardiologiia. 2022 Nov 30;62(11):49-55. doi: 10.18087/cardio.2022.11.n2237.
Aim To study concentrations of adipokines and their associations with proinflammatory cytokines in overweight men with coronary atherosclerosis. Material and methods This study included 79 men aged 45-60 years with atherosclerosis who had undergone coronary endarterectomy during a coronary bypass surgery, and were overweight (body weight index (BWI), 25.0-29.9 kg /m2). Based on a histological analysis of plaques, the patients were divided into two subgroups: 43 men with stable atherosclerotic plaques and 36 men with unstable plaques in coronary arteries. The control group consisted of 40 age- and BWI-matched men without clinical manifestations of IHD. Blood concentrations of adipokines, including adiponectin, adipsin, lipocalin-2, resistin, and plasminogen 1 activator inhibitor were measured by a multiplex analysis with a MILLIPLEX MAP Human Adipokine Panel 1. Concentrations of proinflammatory cytokines, including tumor necrosis factor α (TNF- α), interleukin (IL)-1β, IL-6, and C-reactive protein (CRP) were measured by enzyme immunoassay. Results The blood concentration of lipocalin -2 was higher in patients with coronary atherosclerosis and stable or unstable atherosclerotic plaques than in the control group (p<0.01). Both subgroups of men with coronary atherosclerosis were characterized by significant differences from the control group in concentrations of TNF-α (p<0.05), CRP, and IL-6 (p<0.01). The most significant direct correlations were found between adipokines and TNF-α, IL-6, and CRP (p<0.01). Results of a logistic regression analysis showed that relative odds for the presence of significant coronary stenoses increased with increasing blood concentrations of lipocalin-2 (OR=1.005, 95 % CI: 1.002-1.008, р=0.011) and IL-6 (OR=1.582 , 95 % CI: 1.241-2.017, р=0.001).Conclusion The changes in blood concentrations of adipokines associated with higher levels of proinflammatory cytokines may represent a factor that increases the probability of clinically significant coronary stenosis in overweight men with coronary atherosclerosis.
目的 研究超重男性冠心病患者脂联素等脂肪因子与促炎细胞因子的浓度及其相关性。 材料与方法 本研究纳入 79 名年龄 45-60 岁、接受冠状动脉旁路手术期间行冠状动脉内膜切除术的超重（体重指数（BMI）25.0-29.9kg/m2）男性冠心病患者。根据斑块的组织学分析，将患者分为两个亚组：43 名稳定斑块患者和 36 名冠状动脉不稳定斑块患者。对照组由 40 名年龄和 BMI 匹配、无 IHD 临床表现的男性组成。采用 MILLIPLEX MAP Human Adipokine Panel 1 多重分析测量脂肪因子（包括脂联素、adipsin、lipocalin-2、抵抗素和纤溶酶原 1 激活物抑制剂）的血液浓度。采用酶联免疫吸附试验测定促炎细胞因子（包括肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6 和 C 反应蛋白（CRP））的浓度。 结果 与对照组相比，冠状动脉粥样硬化和稳定或不稳定动脉粥样硬化斑块患者的血 lipocalin-2 浓度更高（p<0.01）。两组冠状动脉粥样硬化男性患者的 TNF-α（p<0.05）、CRP 和 IL-6（p<0.01）浓度均与对照组存在显著差异。脂联素与 TNF-α、IL-6 和 CRP 之间存在最显著的直接相关性（p<0.01）。logistic 回归分析结果显示，随着血 lipocalin-2（OR=1.005，95%CI：1.002-1.008，p=0.011）和 IL-6（OR=1.582，95%CI：1.241-2.017，p=0.001）浓度升高，发生显著冠状动脉狭窄的相对风险增加。 结论 与促炎细胞因子水平升高相关的脂肪因子血浓度变化可能是超重男性冠心病患者发生临床上显著冠状动脉狭窄的概率增加的一个因素。
