Mardon H J, Bee J, von der Mark K, Owen M E
Nuffield Department of Orthopaedic Surgery, Nuffield Orthopaedic Centre, Oxford, United Kingdom.
Cell Tissue Res. 1987 Oct;250(1):157-65. doi: 10.1007/BF00214667.
Diffusion chambers containing bone marrow cells from adult rats were implanted intraperitoneally into rat hosts and cultured in vivo for up to 64 days. Biochemical and histological analyses of the contents of the chambers demonstrate that a connective tissue consisting of bone, cartilage and fibrous tissues is formed by precursor cells present in marrow stroma. The amounts of osteogenic tissue and DNA are directly correlated with time of implantation and with number of cells inoculated. In the chambers there is initial formation of fibrous tissue which is strongly reactive to collagen type III, laminin and fibronectin. In areas of osteogenesis which appear later within this fibrous anlage, expression of collagen type III, laminin and fibronectin decrease and collagen types I and II increase in association with bone and cartilage respectively. Where osteogenesis does not develop, fibrous tissue continues to express collagen type III. The sequential expression of the different extracellular matrix components is similar to that previously observed during osteogenic differentiation in embryonic and adult developmental systems. It is concluded that the formation of fibrous and osteogenic tissues in diffusion chambers by precursor cells present in adult marrow, resembles the normal developmental process.
将含有成年大鼠骨髓细胞的扩散小室腹腔内植入大鼠宿主体内，并在体内培养长达64天。对小室内内容物的生化和组织学分析表明，由骨髓基质中存在的前体细胞形成了一种由骨、软骨和纤维组织组成的结缔组织。成骨组织的量和DNA与植入时间以及接种的细胞数量直接相关。在小室内，最初形成的纤维组织对III型胶原、层粘连蛋白和纤连蛋白有强烈反应。在稍后出现在这种纤维原基内的成骨区域，III型胶原、层粘连蛋白和纤连蛋白的表达减少，而I型和II型胶原分别与骨和软骨相关增加。在没有发生成骨的地方，纤维组织继续表达III型胶原。不同细胞外基质成分的顺序表达与先前在胚胎和成年发育系统的成骨分化过程中观察到的相似。得出的结论是，成年骨髓中存在的前体细胞在扩散小室内形成纤维组织和成骨组织，类似于正常的发育过程。
