Mousavi Seyed Erfan, Hatamipour Mohammad Sadegh, Yegdaneh Afsaneh
Chemical Engineering Department, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
Chemical Engineering Department, Faculty of Engineering, University of Isfahan, Isfahan, Iran.
Int J Biol Macromol. 2023 Jan 31;226:660-669. doi: 10.1016/j.ijbiomac.2022.12.070. Epub 2022 Dec 13.
In this study, the extraction and characterization of alginic acid, the most abundant compound among brown algae were investigated. The used algae were Sargassum angustifolium from the family of brown algae native to the coasts of the Persian Gulf. The effect of temperature, time, algae mass to solvent volume ratio, and ultrasonic power on the extraction yield and ratio of monomers (M/G) was investigated using the central composite design method. Moreover, the effect of the mentioned parameters on the poly dispersity index and cytotoxic effects against breast cancer cells were also investigated. The maximum obtained extraction yield was 46 %, which was higher than those reported for algae in tropical climates. This shows the effectiveness of ultrasound in facilitating the extraction process. In addition, the minimum monomer ratio was 0.45, the minimum poly dispersity index was 2.5 and the maximum cytotoxicity for using the extract on breast cancer cell line (MCF-7) was 20.3 % (with alginic acid concentration of 250 micrograms per milliliter).
在本研究中，对褐藻中最丰富的化合物海藻酸的提取和特性进行了研究。所使用的藻类是来自波斯湾沿岸原生褐藻科的狭叶马尾藻。采用中心复合设计方法，研究了温度、时间、藻体质量与溶剂量之比以及超声功率对提取产率和单体比例（M/G）的影响。此外，还研究了上述参数对多分散指数以及对乳腺癌细胞的细胞毒性作用的影响。获得的最大提取产率为46%，高于热带气候下藻类的报道值。这表明超声在促进提取过程方面的有效性。此外，最小单体比例为0.45，最小多分散指数为2.5，提取物对乳腺癌细胞系（MCF-7）的最大细胞毒性为20.3%（海藻酸浓度为每毫升250微克）。
