Vaseghi Golnaz, Sharifi Mohsen, Dana Nasim, Ghasemi Ahmad, Yegdaneh Afsaneh
Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacognosy, Isfahan Pharmaceutical Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2018 Mar 27;7:43. doi: 10.4103/abr.abr_259_16. eCollection 2018.
Marine organisms produce a variety of compounds with pharmacological activities including anticancer effects. This study attempt to find cytotoxicity of hexane (HEX), dichloromethane (DCM), and butanol (BUTOH) partitions of .
was collected from Bushehr, a Southwest coastline of Persian Gulf. The plant was extracted by maceration with methanol-ethyl acetate. The extract was evaporated under vacuum and partitioned by Kupchan method to yield HEX, DCM, and BUTOH partitions. The cytotoxic activity of the extract (150, 450, and 900 μg/ml) was investigated against MCF-7 (breast cancer), HeLa (cervical cancer), and human umbilical vein endothelial cells cell lines by mitochondrial tetrazolium test assay after 72 h.
The cell survivals of HeLa and MCF-7 cell were decreased by increasing the concentration of extracts from 150 μg/ml to 900 μg/ml. The median growth inhibitory concentration value of HEX partition was 71 and 77 μg/ml against HeLa and MCF-7, dichloromethane partition was 36 and 88 μg/ml against HeLa and MCF-7, respectively. BUTOH partition was 25 μg/ml against MCF-7.
This study reveals that different partitions of have cytotoxic activity against cancer cell lines.
海洋生物产生多种具有药理活性的化合物，包括抗癌作用。本研究试图发现[植物名称未给出]的己烷（HEX）、二氯甲烷（DCM）和丁醇（BUTOH）萃取物的细胞毒性。
[植物名称未给出]采自波斯湾西南海岸线的布什尔。该植物用甲醇 - 乙酸乙酯浸渍提取。提取物在真空下蒸发，并采用库普昌方法进行分离，得到己烷、二氯甲烷和丁醇萃取物。72小时后，通过线粒体四氮唑试验测定提取物（150、450和900μg/ml）对MCF - 7（乳腺癌）、HeLa（宫颈癌）和人脐静脉内皮细胞系的细胞毒性活性。
随着提取物浓度从150μg/ml增加到900μg/ml，HeLa和MCF - 7细胞的存活率降低。己烷萃取物对HeLa和MCF - 7的半数生长抑制浓度值分别为71和77μg/ml，二氯甲烷萃取物对HeLa和MCF - 7的半数生长抑制浓度值分别为36和88μg/ml。丁醇萃取物对MCF - 7的半数生长抑制浓度值为25μg/ml。
本研究表明，[植物名称未给出]的不同萃取物对癌细胞系具有细胞毒性活性。
