来自波斯湾海藻的细胞毒性磺基喹喔啉基甘油
Cytotoxic Sulfoquinovosyl Glycerols from the Seaweed from Persian Gulf.
作者信息
Sajjadi Seyed Ebrahim, Ghobeishavi Sahar, Yegdaneh Afsaneh
机构信息
Department of Pharmacognosy and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Adv Biomed Res. 2024 Mar 28;13:22. doi: 10.4103/abr.abr_103_23. eCollection 2024.
BACKGROUNDS
Seaweeds are an important source of marine organisms that produce a lot of bioactive compounds.
MATERIALS AND METHODS
In this research, the seaweed was collected from Bushehr province of Persian Gulf of Iran. The seaweed was extracted by methanol:ethyl acetate (1:1) using maceration method. The compounds were isolated with different column chromatography and HPLC(High Performance Liquid Chromatography) by silica gel and hexane:ethyl acetate as mobile phase.
RESULTS
The isolated compounds were elucidated structurally by various 1 and 2 D-NMR and MS spectra. Besides the cytotoxicity test was done against HeLa using standard MTT assay and normal cells.
CONCLUSION
It afforded four known sulfoquinovosyl diacylglycerides and fucosterol. Compounds 1-5 showed cytotoxic effects against HeLa and HUVEC cell lines, with IC50 values of 12.2 ± 2.3, 25.8 ± 3.7, 14.9 ± 2.6, 9.8 ± 1.2 μM, and 5.6 ± 1.2, respectively.
背景
海藻是产生大量生物活性化合物的海洋生物的重要来源。
材料与方法
本研究中，海藻采集自伊朗波斯湾的布什尔省。采用浸渍法用甲醇：乙酸乙酯（1:1）对海藻进行提取。通过不同的柱色谱法以及以硅胶为固定相、正己烷：乙酸乙酯为流动相的高效液相色谱法（HPLC）分离化合物。
结果
通过各种一维和二维核磁共振光谱以及质谱对分离得到的化合物进行结构解析。此外，使用标准MTT法对HeLa细胞和正常细胞进行了细胞毒性测试。
结论
得到了四种已知的磺基喹喔啉二酰基甘油和岩藻甾醇。化合物1 - 5对HeLa和人脐静脉内皮细胞系显示出细胞毒性作用，IC50值分别为12.2±2.3、25.8±3.7、14.9±2.6、9.8±1.2 μM和5.6±1.2。
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