Sharma Nimisha, Shivran Mukesh, Singh Narendra, Dubey Anil Kumar, Singh Sanjay Kumar, Sharma Neha, Gupta Ruchi, Vittal Hatkari, Singh Bikram Pratap, Sevanthi Amitha Mithra, Singh Nagendra Kumar
Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Gene Expr Patterns. 2023 Mar;47:119301. doi: 10.1016/j.gep.2022.119301. Epub 2022 Dec 14.
Mango (Mangifera indica L.) is one of the most important commercial fruit crop grown in many parts of the world. Major challenges affecting mango trade are short shelf-life, high susceptibility to chilling injury, post-harvest diseases and consumer demand for improved fruit quality. The objective of the present study was to reveal the key regulators present in bud and flower tissues during flower development stage, associated with fruit development and affect the shelf-life of the mango fruit. RNA-sequencing of contrasting genotypes having short and long shelf-life, was carried out. Comparative differential expression pathway studies of long shelf-life (Totapuri) and short shelf-life (Bombay Green) mango genotypes revealed a total of 177 highly differentially expressed genes. Out of 177 total genes, 101 genes from endoplasmic reticulum pathway and very few from gibberellins (3) and jasmonic acid (1) pathway were identified. Genes from endoplasmic reticulum pathway like hsp 90, SRC2, DFRA, CHS, BG3 and ASPG1 mainly up regulated in Bombay Green. Uniprotein B9R8D3 also shows up regulation in Bombay Green. Ethylene insensitive pathway gene EIL1 up regulated in Bombay Green. Gene CAD1 from phenylpropanoid pathway mainly up regulated in Bombay Green. A total of 4 SSRs and 227 SNPs were mined from these pathways specific to the shelf-life. Molecular studies of endoplasmic reticulum, phenylpropanoid, ethylene, polygalacturonase and hormone pathways at the time of bud and flower formation revealed key regulators that determine the shelf-life of mango fruit.
芒果（Mangifera indica L.）是世界许多地区种植的最重要的商业水果作物之一。影响芒果贸易的主要挑战包括货架期短、对冷害高度敏感、采后病害以及消费者对改善果实品质的需求。本研究的目的是揭示在花发育阶段芽和花组织中存在的与果实发育相关并影响芒果果实货架期的关键调节因子。对具有短货架期和长货架期的不同基因型进行了RNA测序。对长货架期（Totapuri）和短货架期（Bombay Green）芒果基因型的比较差异表达途径研究共发现了177个高度差异表达的基因。在这177个基因中，鉴定出101个来自内质网途径的基因，而来自赤霉素（3个）和茉莉酸（1个）途径的基因很少。内质网途径中的基因，如hsp 90、SRC2、DFRA、CHS、BG3和ASPG1在Bombay Green中主要上调。单蛋白B9R8D3在Bombay Green中也表现出上调。乙烯不敏感途径基因EIL1在Bombay Green中上调。苯丙烷途径中的基因CAD1在Bombay Green中主要上调。从这些与货架期相关的途径中总共挖掘出4个SSR和227个SNP。在芽和花形成时对内质网、苯丙烷、乙烯、多聚半乳糖醛酸酶和激素途径的分子研究揭示了决定芒果果实货架期的关键调节因子。
