从德黑兰污水中分离出的丝状噬菌体对临床耐碳青霉烯菌株的裂解潜力。
Lytic potential of a filamentous bacteriophage isolated from sewage water in Tehran on clinical carbapenem-resistant strains.
作者信息
Esmaeili-Fard-Barzegar Keyvan, Najar-Peerayeh Shahin, Mazaheri Nezhad Fard Ramin, Bakhshi Bita
机构信息
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Microbiol. 2022 Oct;14(5):705-711. doi: 10.18502/ijm.v14i5.10966.
BACKGROUND AND OBJECTIVES
, an opportunistic pathogen, has been isolated from sewage, soil, and hospital wards. The prevalence of multidrug-resistance has seriously caused a health crisis in hospital settings. Bacteriophages have been used as an alternative therapy for control carbapenem-resistant bacteria-caused infections. We aimed to assay lytic effect of a filamentous phage on clinical bacterial strains.
MATERIALS AND METHODS
500-ml water samples was collected from sewage in Tehran. Sewage samples were precipitated at 6000 rpm for 10 minutes and filtered using 0.45-μm syringe filters. Bacteriophage was isolated using double-layer agar assay and evaluated its stability at various pH and temperature ranges. In addition, the stability of the phage was assayed at chloroform 0.1%.
RESULTS
Transmission electron microscopy imagine showed phage is filamentous called vB-AbaI-TMU2. The phage affected on its own host so that could not effect on any and strains. vB-AbaITMU2 phage was stable at pH 5 and 7 and also temperatures 25 and 37°C. vB-AbaI-TMU2 was stable at chloroform 0.1%. vB-AbaI-TMU2 phage infected carbapenem-resistant strains while other bacterial strains were resistant to.
CONCLUSION
The present study indicated the isolated phage had a narrow host range and is susceptible to various pH values and temperatures.
背景与目的
[病原体名称]是一种机会致病菌，已从污水、土壤和医院病房中分离出来。多重耐药性的流行已在医院环境中严重引发了健康危机。噬菌体已被用作控制耐碳青霉烯类细菌引起的感染的替代疗法。我们旨在测定丝状噬菌体对临床细菌菌株的裂解作用。
材料与方法
从德黑兰的污水中采集500毫升水样。污水样本在6000转/分钟下沉淀10分钟，并用0.45微米的注射器滤器过滤。使用双层琼脂试验分离噬菌体，并评估其在不同pH和温度范围内的稳定性。此外，在0.1%的氯仿中测定噬菌体的稳定性。
结果
透射电子显微镜图像显示噬菌体是丝状的，称为vB-AbaI-TMU2。该噬菌体对其自身宿主有影响，但对任何[其他细菌名称]和[其他细菌名称]菌株均无影响。vB-AbaITMU2噬菌体在pH 5和7以及温度25和37°C时稳定。vB-AbaI-TMU2在0.1%的氯仿中稳定。vB-AbaI-TMU2噬菌体感染耐碳青霉烯类[细菌名称]菌株，而其他细菌菌株对其耐药。
结论
本研究表明分离出的噬菌体宿主范围狭窄，且对各种pH值和温度敏感。
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