Kusradze Ia, Karumidze Natia, Rigvava Sophio, Dvalidze Teona, Katsitadze Malkhaz, Amiranashvili Irakli, Goderdzishvili Marina
G. Eliava Institute of Bacteriophages, Microbiology and Virology Tbilisi, Georgia.
G. Eliava Institute of Bacteriophages, Microbiology and VirologyTbilisi, Georgia; Ivane Javakhishvili Tbilisi State UniversityTbilisi, Georgia.
Front Microbiol. 2016 Oct 4;7:1590. doi: 10.3389/fmicb.2016.01590. eCollection 2016.
is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of phage administration in wound infections in an animal model to demonstrate phage therapy as non-toxic, safe and alternative antibacterial remedy. Using classical methods for the study of bacteriophage properties, we characterized phage as a dsDNA myovirus with a 90 kb genome size. Important characteristics of include a short latent period and large burst size, wide host range, resistance to chloroform and thermal and pH stability. In a rat wound model, phage application effectively decreased the number of bacteria isolated from the wounds of successfully treated animals. This study highlights the effectiveness of the phage therapy and provides further insight into treating infections caused by MDR strains using phage administration.
是一种革兰氏阴性、无运动性的细菌，由于其多重耐药性，已成为主要的医院病原体。多重耐药（MDR）菌株数量的增加重新引发了对噬菌体疗法的兴趣。我们研究的目的是评估在动物模型中噬菌体给药对伤口感染的有效性，以证明噬菌体疗法是无毒、安全的替代抗菌疗法。使用研究噬菌体特性的经典方法，我们将噬菌体鉴定为一种基因组大小为90 kb的双链DNA肌病毒。其重要特征包括潜伏期短、裂解量高、宿主范围广、对氯仿有抗性以及热稳定性和pH稳定性。在大鼠伤口模型中，噬菌体的应用有效地减少了从成功治疗动物伤口分离出的细菌数量。这项研究突出了噬菌体疗法的有效性，并为使用噬菌体给药治疗由多重耐药菌株引起的感染提供了进一步的见解。
