Albini Elisa, Orso Massimiliano, Cozzolino Francesco, Sacchini Luca, Leoni Francesca, Magistrali Chiara Francesca
Istituto Zooprofilattico Sperimentale dell'Umbria e Delle Marche 'Togo Rosati', Perugia, Italy.
Health Planning Service, Regional Health Authority of Umbria, Perugia, Italy.
Front Microbiol. 2022 Dec 1;13:1040568. doi: 10.3389/fmicb.2022.1040568. eCollection 2022.
Bivalves are filter-feeding animals able to accumulate contaminants and microorganisms, either of marine or terrestrial origin. The aim of this study was to describe the prevalence of antimicrobial resistance (AMR) in bacterial isolates from bivalves using a systematic review of the literature. Comprehensive searches of MEDLINE, EMBASE, and Web of Science were carried out, based upon a registered protocol (PROSPERO), and following the preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) guidelines. The methodological quality of the included studies was assessed using a modified Hoy checklist. Meta-analyses of prevalence were carried out using random-effects models. In total, 103 articles were selected from 1,280 records and were included in the final analysis. The studies were from Asia ( = 54), Europe ( = 27), South and North America ( = 10 and = 6, respectively), Africa ( = 2), Oceania ( = 1), and multicentre and intercontinental ( = 3). The meta-analysis of multiple antibiotic resistance (MAR) index revealed spp. as the genus with the highest prevalence of AMR (37%), followed by spp. (34%), spp. (18%), and (15%). Resistance to third/fourth/fifth generation cephalosporins and fluoroquinolones, two highest priority, critically important antimicrobials (HPCIA), was recorded in approximately 10% of . isolates. Resistance to carbapenems was very low (<2%) in spp. and in . , but was found in 5% of spp. and in more than a third of spp. isolates. In aquatic bacteria, resistance to carbapenems was higher in Asian than in European isolates. Our study shows the presence of antibiotic resistant bacteria (ARB), including bacteria resistant to HPCIA, in marine bivalves, posing a risk for consumers.
双壳贝类是滤食性动物，能够积累海洋或陆地来源的污染物和微生物。本研究的目的是通过对文献的系统综述来描述双壳贝类细菌分离株中的抗菌药物耐药性（AMR）流行情况。根据注册方案（PROSPERO）并遵循系统评价和Meta分析的首选报告项目（PRISMA）指南，对MEDLINE、EMBASE和科学网进行了全面检索。使用改良的霍伊清单评估纳入研究的方法学质量。使用随机效应模型进行流行率的Meta分析。总共从1280条记录中选择了103篇文章并纳入最终分析。这些研究来自亚洲（n = 54）、欧洲（n = 27）、南美洲和北美洲（分别为n = 10和n = 6）、非洲（n = 2）、大洋洲（n = 1）以及多中心和洲际研究（n = 3）。多重抗生素耐药性（MAR）指数的Meta分析显示，弧菌属是AMR流行率最高的属（37%），其次是芽孢杆菌属（34%）、不动杆菌属（18%）和肠杆菌属（15%）。在大约10%的弧菌属分离株中记录到对第三代/第四代/第五代头孢菌素和氟喹诺酮类这两种最高优先级、至关重要的抗菌药物（HPCIA）的耐药性。在芽孢杆菌属和不动杆菌属中，对碳青霉烯类的耐药性非常低（<2%），但在弧菌属的5%以及肠杆菌属超过三分之一的分离株中发现了这种耐药性。在水生细菌中，亚洲分离株对碳青霉烯类的耐药性高于欧洲分离株。我们的研究表明，海洋双壳贝类中存在抗生素耐药菌（ARB），包括对HPCIA耐药的细菌，这对消费者构成了风险。
