Kurosawa A, Elner V M, Yue B Y, Elvart J L, Tso M O
Department of Ophthalmology, University of Illinois at Chicago 60612.
Exp Eye Res. 1987 Aug;45(2):239-51. doi: 10.1016/s0014-4835(87)80147-1.
We examined the extracellular matrix formation and lectin-binding properties of cultured trabecular-meshwork cells established from cynomolgus monkey and bovine eyes. Using an avidin-biotin complex method, we found that the extracellular matrix in both the monkey and bovine cultures stained intensely with antibodies to fibronectin, type IV collagen, and laminin. These materials were especially prominent when the cultured monkey trabecular-meshwork cells aggregated in clusters, manifesting their close anatomic relationship to trabecular beams. Cell-surface and intracellular lectin-binding properties were studied using eight biotinylated lectins: concanavalin A (Con A), peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Dolichos biflorus agglutinin (DBA), soy bean agglutinin (SBA), Phaseolus vulgaris (PHA), wheat germ agglutinin (WGA), and Ulex europaeus agglutinin I (UEA I). Both monkey and bovine trabecular-meshwork cells showed positive cell surface and intracellular binding to Con A, WGA, and PHA. Only moderate cell-surface staining was observed with RCA, and no visible staining occurred with PNA, DBA, SBA, and UEA-1.
我们研究了从食蟹猴和牛眼建立的培养小梁网细胞的细胞外基质形成和凝集素结合特性。使用抗生物素蛋白-生物素复合物方法，我们发现猴和牛培养物中的细胞外基质均被抗纤连蛋白、IV型胶原和层粘连蛋白抗体强烈染色。当培养的猴小梁网细胞聚集成簇时，这些物质尤为突出，表明它们与小梁束有密切的解剖关系。使用八种生物素化凝集素研究细胞表面和细胞内凝集素结合特性：伴刀豆球蛋白A（Con A）、花生凝集素（PNA）、蓖麻凝集素（RCA）、双花扁豆凝集素（DBA）、大豆凝集素（SBA）、菜豆凝集素（PHA）、麦胚凝集素（WGA）和荆豆凝集素I（UEA I）。猴和牛小梁网细胞均显示细胞表面和细胞内与Con A、WGA和PHA呈阳性结合。用RCA仅观察到中度细胞表面染色，而用PNA、DBA、SBA和UEA-1未观察到可见染色。
