感染伯氏考克斯体的豚鼠体内的抗原特异性循环免疫复合物

Antigen-specific circulating immune complexes in Coxiella burnetii-infected guinea pigs.

作者信息

Wen B H, Yu S R

机构信息

Department of Microbiology, Third Military Medical College, Chongqing, China.

出版信息

Exp Mol Pathol. 1987 Oct;47(2):175-84. doi: 10.1016/0014-4800(87)90072-4.

DOI:10.1016/0014-4800(87)90072-4

PMID:3653345

Abstract

An antigen-specific conglutinin-binding assay was developed with artificial immune complexes of Coxiella burnetii and an enzyme-linked immunosorbent assay for the detection of circulating immune complexes. Sera from guinea pigs infected with C. burnetii were examined by this assay, and the percentages of infected guinea pigs with C. burnetii antigen-specific circulating immune complexes were 71, 93, 74, 65, 49, and 27%, respectively, from the first to sixth week after infection. C. burnetii antigen eluted from circulating immune complexes by acid dissociation was detected by counterimmunoelectrophoresis. The antigen-specific conglutinin binding assay was specific, sensitive, and reproducible for assay of circulating immune complexes.

摘要

开发了一种针对伯氏考克斯氏体人工免疫复合物的抗原特异性胶固素结合试验以及一种用于检测循环免疫复合物的酶联免疫吸附测定法。用该试验检测感染伯氏考克斯氏体的豚鼠血清，感染后第一至六周感染伯氏考克斯氏体且具有抗原特异性循环免疫复合物的豚鼠百分比分别为71%、93%、74%、65%、49%和27%。通过酸解离从循环免疫复合物中洗脱的伯氏考克斯氏体抗原用对流免疫电泳进行检测。抗原特异性胶固素结合试验对于循环免疫复合物的检测具有特异性、敏感性和可重复性。