维生素 D 与 IFN-γ、NF-κB、甲状腺自身抗体水平在唐氏综合征中的相关性：印度尼西亚儿童的研究。

The correlation between vitamin D and levels of IFN-γ, NF-κB, thyroid antibodies in down syndrome: study in Indonesian children.

机构信息

Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia; Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia.

Faculty of Medicine, Department of child health, Dr. Soetomo General Hospital, Universitas Airlangga, Surabaya, East Java, Indonesia.

出版信息

Acta Biomed. 2022 Dec 16;93(6):e2022342. doi: 10.23750/abm.v93i6.13722.

DOI:10.23750/abm.v93i6.13722

PMID:36533745

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9828918/

Abstract

BACKGROUND AND AIM

Vitamin D (VD) reduces interferon-gamma (IFN-γ) production and prevents nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, impacting the inhibition of the autoimmunity process such as autoimmune thyroiditis (AITD). Children with Down syndrome (DS) are reported to have a higher risk of autoimmunity and lower VD levels than non-DS. Therefore, this study aimed to evaluate VD levels in Indonesian DS children and their relationship with marker of AITD.

METHODS

This study was conducted on DS children at Dr Soetomo Hospital between February 2021-June 2022. Socio-demographic status, amount of milk, fish and meat consumption, and duration of sun exposure were obtained using a self-report questionnaire. Thyroid hormone (TSH and FT4), thyroid antibody (TPO-Ab and Tg-Ab), 25 (OH)D, IFN-γ, and NF-κB levels were measured using ELISA.

RESULTS

Of the 80 participants, 53.75% had sufficient (50.829±17.713 ng/ml) and 46.25% had non-sufficient (20.606±5.974 ng/ml) VD levels. Daily milk consumption, meat and fish consumption were risk factors contributing to VD levels in multivariate analysis [p=0.003, OR=1.007(1.003-1.012); p=0.004, OR=1.816(1.209- 2.728), respectively]. Participants with sufficient VD had significantly higher TPO-Ab (p=0.007) and Tg-Ab (p=0.016). Mean of VD levels were significantly negatively correlated with IFN-γ levels (r =-0.262, p=0.037) and positively correlated with TPO-Ab (r= 0.432, p=1x10-5,) and Tg-Ab (r= 0.375, p=0.001).

CONCLUSIONS

Majority of subjects had sufficient VD levels. VD suppresses IFN-g, but is unable to affect NF-κB levels, presumably causing high levels of TPO-Ab and Tg-Ab in sufficient VD patient.

摘要

背景与目的

维生素 D（VD）可减少干扰素-γ（IFN-γ）的产生并抑制核因子 κB 轻链增强子的活性 B 细胞（NF-κB）的激活，从而影响自身免疫过程的抑制，如自身免疫性甲状腺炎（AITD）。据报道，唐氏综合征（DS）患儿发生自身免疫的风险较高，VD 水平较低。因此，本研究旨在评估印度尼西亚 DS 儿童的 VD 水平及其与 AITD 标志物的关系。

方法

本研究于 2021 年 2 月至 2022 年 6 月在苏托莫医院对 DS 患儿进行。采用自报问卷获取社会人口统计学状况、牛奶、鱼和肉的摄入量以及日照时间。使用 ELISA 法测量甲状腺激素（TSH 和 FT4）、甲状腺抗体（TPO-Ab 和 Tg-Ab）、25（OH）D、IFN-γ 和 NF-κB 水平。

结果

80 名参与者中，53.75%（50.829±17.713ng/ml）VD 水平充足，46.25%（20.606±5.974ng/ml）VD 水平不足。多变量分析显示，每日牛奶摄入量、肉类和鱼类摄入量是影响 VD 水平的危险因素[分别为 p=0.003，OR=1.007（1.003-1.012）；p=0.004，OR=1.816（1.209-2.728）]。VD 充足的参与者 TPO-Ab（p=0.007）和 Tg-Ab（p=0.016）显著较高。VD 水平均值与 IFN-γ水平呈显著负相关（r=-0.262，p=0.037），与 TPO-Ab（r=0.432，p=1x10-5）和 Tg-Ab（r=0.375，p=0.001）呈显著正相关。