Nordlander I, Johnsson H, Osterdahl B
Nutrition Laboratory, Swedish National Food Administration, Uppsala, Sweden.
Food Addit Contam. 1987 Jul-Sep;4(3):291-6. doi: 10.1080/02652038709373638.
A rapid and sensitive HPLC method was developed for the determination of oxytetracycline in fish tissues (muscle and liver) based on a clean-up and concentration procedure on Sep-Pak C18. At a coastal fishfarm rainbow trout (Salmo gairdneri) suffering from Vibrio anguillarum were treated with 75 mg oxytetracycline per kg fish and day for ten days. Oxytetracycline residues above the limit of determination (0.005 micrograms/g) were found in fish 82 days after treatment. The recoveries from spiked tissues were about 60% and 70% for muscle and liver, respectively.
建立了一种快速灵敏的高效液相色谱法，用于测定鱼组织（肌肉和肝脏）中的土霉素，该方法基于Sep-Pak C18柱的净化和浓缩步骤。在一个沿海养鱼场，对感染鳗弧菌的虹鳟鱼（Salmo gairdneri）以每千克鱼每天75毫克土霉素的剂量治疗10天。治疗82天后，在鱼体内发现土霉素残留量超过测定限（0.005微克/克）。加标组织中肌肉和肝脏的回收率分别约为60%和70%。
