Grover S K, Srivastava K K, Misra U K
Department of Biochemistry, V.P. Chest Institute, University of Delhi, India.
Int J Vitam Nutr Res. 1987;57(2):115-9.
The hepatic MFO activity has been studied in rats exposed to acute hypoxia given single dose of ethanol (3 g/kg body weight) alone or along with 10,000 I.U. of vitamin A. The cytochrome P-450 was found to be depressed on ethanol administration in rats. Hypoxic exposure of rats given ethanol with or without vitamin A, however, restored the depressed cytochrome P-450 activity. Cytochrome-c-reductase, on the other hand, was increased on administration of ethanol with or without vitamin A supplementation. Exposure to hypoxia did not have any additional effect on cytochrome c-reductase activity. Aminopyrine-N-demethylase was depressed on ethanol administration. Vitamin A administration further depressed this enzyme. Hypoxic exposure, however, did not restore this enzyme level. Aniline hydroxylase was not affected by ethanol administration, vitamin A supplementation and hypoxic exposure. Thus, mixed function oxidase system appears to be differently affected on ethanol administration, vitamin A supplementation and hypoxic exposure suggesting that these effectors have different loci of interaction in microsomal structure and/or in its micromileau of cofactors.
已经在单独给予单剂量乙醇（3克/千克体重）或同时给予10,000国际单位维生素A的急性缺氧大鼠中研究了肝脏微粒体混合功能氧化酶（MFO）活性。发现给予乙醇后大鼠体内的细胞色素P-450水平降低。然而，给予乙醇的大鼠无论是否补充维生素A，缺氧暴露都能恢复降低的细胞色素P-450活性。另一方面，无论是否补充维生素A，给予乙醇后细胞色素c还原酶都会增加。缺氧暴露对细胞色素c还原酶活性没有任何额外影响。给予乙醇后氨基比林-N-脱甲基酶活性降低。给予维生素A会进一步降低这种酶的活性。然而，缺氧暴露并没有使这种酶的水平恢复。给予乙醇、补充维生素A和缺氧暴露对苯胺羟化酶均无影响。因此，混合功能氧化酶系统在给予乙醇、补充维生素A和缺氧暴露时似乎受到不同的影响，这表明这些效应物在微粒体结构和/或其辅因子微环境中有不同的相互作用位点。
