改良的水凝胶制备方案以降低细胞含量。
A modified hydrogel production protocol to decrease cellular content.
机构信息
Bachelor. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP), Brazil.
PhD. Universidade de São Paulo - Discipline of Plastic Surgery, Microsurgery and Plastic Surgery Laboratory - School of Medicine - São Paulo (SP), Brazil.
出版信息
Acta Cir Bras. 2022 Dec 19;37(10):e371005. doi: 10.1590/acb371005. eCollection 2022.
PURPOSE
To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix.
METHODS
We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation.
RESULTS
After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days.
CONCLUSIONS
The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.
目的
分析猪源性去细胞真皮基质的细胞毒性和细胞。
方法
我们通过组织学分析、DNA 定量和流式细胞术分析了多种去细胞化过程的效果。随后，我们分析了最合适的水凝胶浓度，以最大限度地减少对成纤维细胞培养的细胞毒性，并最大限度地提高细胞增殖。
结果
经过第四次去细胞化后，DNA 定量显示 DNA 浓度最低（<50ng/mg）。组织学分析显示水凝胶中没有细胞成分。此外，苏木精和伊红显示出胶原纤维的异质结构。最佳水凝胶浓度范围为 3%至 25%，24 小时和七天之间没有显著差异。
结论
水凝胶制备过程对去除细胞和 DNA 元素有效。最佳水凝胶浓度范围为 3%至 25%。
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