猪心组织的去细胞化以获得基于细胞外基质的水凝胶。
Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels.
机构信息
Complex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regeneration Medicine, Maastricht University, Maastricht, The Netherlands; Department of Biomedical Engineering and Sciences, School of Mechanical and Manufacturing Engineering, National University of Sciences and Technology (NUST), Islamabad, Pakistan.
Complex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regeneration Medicine, Maastricht University, Maastricht, The Netherlands; Department of Pulmonology and Physiology, Vrije Universiteit University Medical Center, Amsterdam, The Netherlands.
出版信息
Methods Cell Biol. 2020;157:3-21. doi: 10.1016/bs.mcb.2019.11.023. Epub 2019 Dec 31.
The use of hydrogels derived from the extracellular matrix (ECM) in tissue engineering applications aims to overcome the conundrum of mimicking the complexity of ECM composition in vitro. In this chapter, we describe a method of decellularization and subsequent formation of an ECM-based hydrogel using porcine heart tissue. These decellularized ECM hydrogels could be used to create semi-interpenetrating networks or as bioinks in bioprinting applications to further enhance the bioactivity and increase the biomimicry degree of the biological cardiac constructs.
在组织工程应用中使用源自细胞外基质（ECM）的水凝胶旨在解决在体外模拟 ECM 组成复杂性的难题。在本章中，我们描述了一种使用猪心组织进行脱细胞和随后形成基于 ECM 的水凝胶的方法。这些脱细胞 ECM 水凝胶可用于创建半互穿网络或用作生物打印应用中的生物墨水，以进一步提高生物活性并增加生物心脏构建体的仿生程度。
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