Department of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 770-8503, Japan.
Department of Oral and Maxillofacial Anatomy, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, 770-8503, Japan.
Sci Rep. 2020 Feb 3;10(1):1656. doi: 10.1038/s41598-020-58222-0.
Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
牙周组织是一种独特的三维组织结构，由牙骨质、牙周韧带（PDL）和牙槽骨组成。严重的牙周病会对口腔功能和整体健康造成根本问题，而传统的牙科治疗不足以使牙周组织恢复健康。细胞片技术已被用于许多组织再生，包括牙周组织，将合适的干细胞/祖细胞移植到目标部位作为一个统一的组织，以进行组织再生。然而，仍然难以构建由多种类型的细胞组成的复杂组织的三维结构，并且单个细胞片的移植不能充分再生大规模的组织损伤。在这里，我们通过在温度响应培养皿上分层牙周韧带细胞和成骨样细胞，制造了由骨-韧带结构组成的三维复杂细胞片。异位和原位移植后，只有复杂的细胞片组被证明能够沿着牙周韧带样纤维与牙根和牙槽骨的功能连接，解剖学上再生骨-韧带结构。这项研究代表了使用模拟解剖结构的生物工程组织成功地进行了大规模组织损伤的三维组织再生。
